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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
见包装
- 保质期:
见包装
- 英文名:
Agarose Gel DNA Extraction and Purification
- 库存:
999
- 供应商:
博奥派克
- 规格:
100 Tests
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文献和实验Materials: agarose solution in TBE or TAE (generally 0.7-1%) 1X TBE or TAE (same buffer as in agarose) gel loading dye
Extraction of DNA from Agarose Gels
, to destabilize the agarose gel. The DNA is subsequently bound to a substrate (e.g., an anionic resin) and washed to remove impurities prior to elution of the DNA from the substrate. Other methods include hot phenol extraction of the DNA from the gel. The use
DNA fingerprinting - agarose gel
. After digestion, a brief centrifugation will collect DNA at the bottom of wells. Seal plates with foil tape and store at 4℃ if necessary. Prepare 1% agarose gels in 1X TAE: Cool molten agarose to 46℃ in a water bath with occasional stirring. Pour into 20X25 cm UV
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