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文献和实验of choice (15µg, Lipofectamine 2000™ Invitrogen). The following day the flag-tagged protein expressing plasmid transfected cultures are transfected again with 100nM EF52 biotin labeled siRNA (antisense or sense alone) using Lipofectamine 2000™ or stock 3.4µM
expressing plasmid of choice (15µg, Lipofectamine 2000™ Invitrogen). The following day the flag-tagged protein expressing plasmid transfected cultures are transfected again with 100nM EF52 biotin labeled siRNA (antisense or sense alone) using Lipofectamine
Purification of Human Multiprotein Complexes using OneSTrEP Technology (PROT41)
. Elution of the complexes by competition with D-biotin is efficient and can be done in a variety of buffer conditions allowing biochemical active complexes to be preserved. For more theoretical background and a protocol for purification of recombinant
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