Specificity Note: HSV 2, specific for gD. Functions in western blot, ELISA & IFA. Conjugation: FITC CONJ Fluorescein Conjugates: These MONOTOPE products consist of purified monoclonal antibody conjugated with high purity isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product. The final preparation is formulated to an antibodyconcentration of of 100 ?g/ml in 0.01 M phosphate buffered saline, pH 7.2 containing 0.1% sodium azide plus bovine serum albumin at 10 mg/ml. Each vial contains 1.0 ml. This product should be stored at -20C until ready for use. Avoid repeated freeze-thawing by storing multiple aliquots at -20C. Applications for these products include direct FA staining of target antigen in a permissive tissue culture system. Working strength must be determined by the user for each specific application but a starting range of 1:5 - 1:20 is recommended. Acetone fixation of the antigen source is recommended prior to staining.