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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
见包装
- 保质期:
见包装
- 英文名:
Chromogenic substrate for plasmin【D-VLK-pNA-25 mg】
- 库存:
999
- 供应商:
博奥派克
- 规格:
25 mg
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文献和实验beta-galactosidase gene lacZ is frequently used as a reporter gene. The expression of transgenic constructs can be monitored by histochemistry with the chromogenic substrate X-gal. This allows precise cellular localization of gene activity
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
1954, Pearson et al 1963). The most common such substrate is an indole derivative, 5-bromo-4-chloro-3-indolyl-ß-D-galactoside (X-gal, Holt and Sadler, 1958). When ß-gal cleaves the glycosidic linkage in X-gal, a soluble, colorless indoxyl monomer
at the determination of the antibody binding to the coated antigen. 7. Develop the ELISA with a suitable chromogenic, fluorogenic or chemiluminescent substrate, and measure the individual wells with an appropriate ELISA plate reader. The highest ELISA signal
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