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- HTAQ-500 units
- 2025年09月03日
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- 保存条件：
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- 英文名：
TaqTivate Hot Start Taq DNA Polymerase【HTAQ-500 units】
- 库存：
999
- 供应商：
博奥派克
- 规格：
500 units
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文献和实验Home-made Taq Polymerase Purification
Home-made Taq Polymerase Purification I also have the bacteria containing the clone. It appears to produce lots of Taq and is quite stable. The proceedure takes 4 days start to (15 000 units of Taq) finish. The Taq also appears
Single tube confirmation PCR protocol
until the PCR is started. Alternatively, a "hot start" can be performed by adding the Taq polymerase to the individual tubes after the PCR mixtures have been heated to 94°C. Hot start improves the PCR results but this step is labor intensive
of 0.2 µM. Add H2O to a final volume of 25 µl (including the volume of Taq DNA polymerase to be added in Step 6). Add 5 units of Taq DNA polymerase. Amplify for 35-40 cycles in a thermal cycler, choosing an appropriate annealing temperature
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