4C for short term (weeks) and -20C for long term. Avoid frequent freeze and thaw.
克隆性:
单克隆
适应物种:
Human, Mouse, Rat
库存:
大量
供应商:
genway
应用范围:
IHC, WB
抗体英文名:
Anti- p70 S6 Kinase (Ab-411) Antibody
Binding Site: around Ser411 WB Dilution: 500~1000
IHC Dilution: 50~100
Storage/Stability: Sore at -20C/1 year Form of Antibody: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human p70 S6 Kinase around the phosphorylation site of serine 411 (I-R-SP-P-R).
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity/Sensitivity: p70 S6 Kinase (Ab-411) antibody detects endogenous levels of total p70 S6 Kinase protein.
Applications: WB: 1:500~1:1000 IHC: 1:50-1:100 IF:1:100~1:200 Function: Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens.
Catalytic Activity: ATP + a protein = ADP + a phosphoprotein.
Enzyme Regulation: Activation by serine/threonine phosphorylation and protein kinase C, inactivated by type 2A phosphatase.
Subunit: Interacts with PPP1R9A/neurabin-1 (By similarity).
Similarity: Contains 1 protein kinase domain. [1] Grove J., Bonerjee P., Balasubramanyam A., Coffer P., Price D.J., Avruch J., Woodgett J.R.
Cloning and expression of two human p70 S6 kinase polypeptides differing only at their amino termini. [2] Gout I., Minami T., Hara K., Tsujishita Y., Filonenko V., Waterfield M.D., Yonezawa K.
Molecular cloning and characterization of a novel p70 S6 kinase, p70 S6 kinase beta containing a proline-rich region. [3] Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. [4] Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
A probability-based approach for high-throughput protein phosphorylation analysis and site localization.