The lysate is shipped with dry ice. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles. <br>Lysate samples can be diluted with 2xSDS Sample Buffer p
Application: Western Blot experiments.
Left-Control; Right -Over-expression Lysate Description: Antigen standard for peroxisomal biogenesis factor 19 (PEX19), transcript variant 1 (transient overexpression lysate)
Components: 1 vial of 100 ug gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 ug empty vector transfected control cell lysate in RIPA buffer
1 vial of 0.5 ml 2xSDS Sample Buffer (4%SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 5% b-mercaptoethanol) Altered Expression of Regulators of the Cortical Chloride Transporters NKCC1 and KCC2 in Schizophrenia Arch Gen Psychiatry, Sep 2010; 10.1001/archgenpsychiatry.2010.114 [STK39]
Coxsackievirus B3 Infection Activates the Unfolded Protein Response and Induces Apoptosis through Downregulation of p58IPK and Activation of CHOP and SREBP1 J. Virol., Sep 2010; 84: 8446 - 8459 [ATF6a]
Elevated cyclic AMP (cAMP) levels in T lymphocytes transformed by human T-cell lymphotropic virus type 1 (HTLV-1) J. Virol., Sep 2010; 84: 8732 - 8742 [PDE3B]