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Standard neutral agarose electrophoresis

  Standard neutral agarose electrophoresis Standard agarose gels can be prepared using either TBE or TAE running buffers. You will need: Either 10 x TBE or 10 x TAE buffers 1 x TBE buffer - 89mM Tris, 89mM boric acid, 2mM EDTA, pH

De-phosphorylation of DNA

The preferred method of de-phosphorylation uses the buffer system described by Pharmacia for most of their restriction endonucleases. You will need: 10 x OPA+ Buffer (100mM Tris.acetate, pH 7.5, 100mM magnesium acetate, 500mM potassium acetate

Single Primer ("Semi-Random") P

to about 1 ng/µL 3 µL "T/TS" @ 0.4 u Taq/µL -------- 30 µL Cycle as follows: 30"x94°C 20 cycles 0"x94°C 0"x55°C 1'x72° S=9 30 cycles 0"x94°C 0"x40°C 1'x72° S=6 30 cycles 0"x94°C 0"x55°C 1'x72° S=9 Clean up: Add 1 µL ExoI