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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
\N
- 保质期:
二年
- 英文名:
(3-Amino-pyridin-4-yl)-acetic acid
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
878483-87-5
- 规格:
5mg/10mg/25mg/50mg
| 规格: | 5mg | 产品价格: | ¥600.0 |
|---|---|---|---|
| 规格: | 10mg | 产品价格: | ¥900.0 |
| 规格: | 25mg | 产品价格: | ¥1600.0 |
| 规格: | 50mg | 产品价格: | ¥3000.0 |
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文献和实验Protocol for Trichloroacetic Acid (TCA) Precipitation
and Equipment Required Borosilicate tubes, 12 x 75 mm (e.g., Fisher Cat #14-961-26) 10% TCA (e.g., Acros Cat # 42145-5000; make a 10% (w/v) solution in water) Warning: TCA is caustic! Carrier nucleic acid, 1 mg/ml (e.g., sheared fish
with ITS (4 ml/L should yield 5 mg/L insulin and 5 mg/L transferrin). --> "Appropriate" = bring cells to a final concentration of 5 X 105 cells/ml. 4) Add [3H] arachidonic acid to get ~2 µCi/ml (2 X 106 µCi/ml???). --> Make sure final AA
Arachidonic Acid Labeling Contributor: Suprya Jayadev Date: November 4, 1991 1) Grow cells to a density of 5-8 X 105 cells/ml in RPMI 1640 containing serum. 2) Pellet cells and wash 1 time with room temperature PBS
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