Introduction The study of gene expression often needs RNA preparation followed by cDNA synthesis and PCR.
However, difficulty in obtaining a large amount of cells for RNA preparation is the bottleneck in some of the studies, such as laser dissected samples, FACS sorted cells, the cultured cells in 96-wells, and liquid biopsy.Cell number is limited (<2,000 cells), which restrict RNA preparation. Signosis’ Direct cDNA cell lysis buffer
allows preparation of cell lysate, which can be used for direct reverse transcription without RNA preparation. Cell lysate made from a few cells is good enough for reverse transcription of RNA to cDNA, and following traditional PCR and real time PCR analysis or cDNA plate array
assays.
Materials provided 10ml Direct cDNA cell lysis buffer (-20ºC)