- ￥5600
- GeneTex
- 美国
- GTX00957
- 2025年07月06日
- WB, ICC/IF, IHC-P
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthesized peptide derived from human Phospho-Histone H3 (S10).
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX00957
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
Histone H3S10ph (phospho Ser10)
- 抗体英文名：
Histone H3S10ph (phospho Ser10) antibody [GT1195]
- 抗体名：
Histone H3S10ph (phospho Ser10) 抗体 [GT1195]
- 规格：
100 μl
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H3S10ph (phospho Ser10) antibody [GT1195] (GTX00957) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
IHC-P analysis of human oophoroma tissue section using GTX00957 Histone H3S10ph (phospho Ser10) antibody [GT1195].
Dilution : 1:100
WB analysis of NIH-3T3 cell extracts with/without nocodazole (50 ng/ml, 37ºC, 20 hrs) treatment or CIP (20μl/400μl, 37ºC, 1 hr) treatment using GTX00957 Histone H3S10ph (phospho Ser10) antibody [GT1195].
Dilution : 1:1000
Loading : 25μg
ICC/IF analysis of PFA-fixed NIH-3T3 cells using GTX00957 Histone H3S10ph (phospho Ser10) antibody [GT1195].
Orange : Primary antibody
Blue : DAPI
WB analysis of C6 cell extracts with/without nocodazole (50 ng/ml, 37ºC, 20 hrs) treatment or CIP (20μl/400μl, 37ºC, 1 hr) treatment using GTX00957 Histone H3S10ph (phospho Ser10) antibody [GT1195].
Dilution : 1:1000
Loading : 25μg
WB analysis of HeLa cell extracts with/without nocodazole (50 ng/ml, 37ºC, 20 hrs) treatment or CIP (20μl/400μl, 37ºC, 1 hr) treatment using GTX00957 Histone H3S10ph (phospho Ser10) antibody [GT1195].
Dilution : 1:1000
Loading : 25μg
ICC/IF analysis of PFA-fixed C6 cells using GTX00957 Histone H3S10ph (phospho Ser10) antibody [GT1195].
Orange : Primary antibody
Blue : DAPI
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文献和实验IMMUNOSCREENING OF LAMBDA-GT11 BACTERIOPHAGE LIBRARIES WITH ANTIBODY PROBES
ml of alkaline phosphatase buffer. Soak membrane sections in this solution for 5 - 10 minutes while mixing. Keep this mixture under foil to reduce exposure to light. Library Screening: Once the serum is sufficiently adsorbed, a lambda-gt11
by adding 1µl Rnase A (10mg/ml) and 20µl of 5M NaCl. Incubate the samples in a 65° water bath or heat block for 4-5 hours to overnight (note 5); ;After the reverse cross-linking the elutes are treated with 10µl of 0.5M EDTA, 20µl of 1M Tris-HCL, pH
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
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