- ￥5900
- GeneTex
- 美国
- GTX00935
- 2025年11月03日
- WB, ELISA, EM
- Rabbit
- Arabidopsis thaliana
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthetic peptide (C)AARAVSLQGQHGPFQSRKIY, whose sequence is derived from the large subunit of Arabidopsis 2S3 albumin
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Arabidopsis thaliana
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Arabidopsis thaliana
- 目录编号：
GTX00935
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ELISA, EM
- 浓度：
2 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
It reacts with the precursor and large subunit of Arabidopsis thaliana 2S albumin.
- 抗体英文名：
2S3 albumin antibody
- 抗体名：
2S3 albumin 抗体
- 规格：
200 μg
WB analysis of Arabidopsis dry seeds extract using GTX00935 2S3 albumin antibody.
p2S: Precursor of 2S
2S-L : Large subunit of 2S
Lane 1 : Dry seeds of wildtype
Lane 2-4 : mag2-2: Dry seeds Mutants that accumulate the precursors of major storage proteins (mag5-1, mag2-1, and mag4-1)
Dilution : 1:2500
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文献和实验environment with little denaturation. Purified antibodies: Once purified, if the antibody is at less than 0.1 mg/ml add a small amount of detergent or protein (0.1% TWEEN-20 or 1% bovine serum albumin) and some protease inhibitors to the solution
volumes running buffer (the serum albumin should stick to the column and the Ig should flow through).(6) On ice slowly add saturated ammonium sulfate to 45% (550 ml sample 450 ml saturated ammonium sulfate). Tilt overnight at 4°C.(7) Pellet by spinning
Antibody Purification This protocol includes an ammonium sulfate cut, affigel blue chromatography and affinity chromatography. Solutions Affigel Blue Prewash 0.1 M acetic acid 5.7 ml glacial acetic acid
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