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- 技术资料
- 保存条件:
4°C, protect from light
- 库存:
货期:1-2天
- 供应商:
MedChemExpress LLC
- CAS号:
84166-13-2
- 规格:
25 mg/50 mg/100 mg
| 规格: | 25 mg | 产品价格: | ¥545.0 |
|---|---|---|---|
| 规格: | 50 mg | 产品价格: | ¥875.0 |
| 规格: | 100 mg | 产品价格: | ¥1400.0 |
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Cibacron Blue 3G-A
CAS No. : 84166-13-2
MCE 国际站:Cibacron Blue 3G-A
产品活性:Cibacron Blue 3G-A 是一种蒽醌染料,可以抑制 R46 β-lactamase,Ki 值为 1.2 uM。
研究领域:Others
作用靶点:Fluorescent Dye
In Vitro: Cibacron Blue 3G-A is a structural analogy between the dye and NADH, it interacts with (di)nucleotide-dependent enzymes and can be a standard tool for studying their active sites.
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文献和实验Blue Native Gel Electrophoresis
Blue Native Gel Electrophoresis Stock solutions 49.5%T, 3%C Acrylamide 24 g acrylamide, 0.75 g bisacrylamide / 50 ml H2O Store at RT 3 x Gel buffer 150 mM BisTris-HCl, 1.5 M 6-amino-caproic acid, pH 7.0 Adjust pH to 7.0 with HCl at 4°C
(%) f Stock solution g 11 13 14 16 18 20 H 2 O h 2.72 ml 2.37 ml 2.20 ml 1.85 ml 1.50 ml 1.15 ml Heavy gel buffer 2.33 ml 2.33 ml
(Cibacron Blue F3G-A coupledto Sepharose), ~100 mL packed into an empty column with100–200 mL of bed volume. 3. Binding Buffer: 1% (w/v) CHAPS, 150 mM KCl, and 20 mMTris-HCl, pH 7.5, sterile filtered. 4. Elution Buffer: 1% (w/v) CHAPS, 1.5 M KCl
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