RiboReady™ 1 kb RNA Ladder provides precise sizing markers for RNA molecules resolved on denaturing or native agarose gels. The ladder offers 9 bands ranging from 200 to 4,000 bases. Each kit provides sufficient material for 50×10µl loads of RNA markers.
Each kit contains:
2 tubes of RNA Ladder lyophilized powder (Each tube provides sufficient material for 25 lanes)
1 tube of 1× loading (reconstitution) buffer
1 tube of 2× loading buffer
Each tube of the lyophilized RNA markers is reconstituted in 250 µl of 1× loading buffer. The 2× loading buffer is added to each RNA sample for consistency within the gel and reproducible results. Tracking dyes are included in both loading buffers to monitor the progression of electrophoresis. The loading buffers also contain ethidium bromide as an RNA stain for immediate viewing of RNA bands with a standard u.v. transilluminator after electrophoresis.