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文献和实验LABELING GIZZARD MYOSIN WITH IATR (MHC AND 17KD MLC)
Day 1-2 Materials 1.0.5 M KCl,10 mM HEPES,pH 7.5,4℃,250 ml. 2.0.5 M KCl,50 mM HEPES,pH 8.0,4℃,250 ml. 3.Bio-Beads SM-2 in a 0.7x15 column. 4.20 mM KCl,20 mM PIPES,pH 7.0,4℃,250 ml. 5.IATR (tetramethylrhodamine iodoacetamide; Molecular Probes
Dynabeads® mRNA DIRECT Micro Kit - mRNA isolation for RT-PCR amplification
and discard the supernatant. 12) Resuspend the Dynabeads-mRNA complex in 100 μl Washing Buffer B. 13) Place the sample tube on the magnet and discard the supernatant. 14) Remove the sample tube from the magnet and resuspend in 100 μl
In Situ Hybridization Using Digoxigenin Labeled Probes
and SpeedVac dry for 2 min 7) Resuspend pellet in 75 ul hybridization buffer. Hybridization Buffer 0.6 M NaCl 50 mM Sodium phosphate buffer, pH 6.8 1X Denhardt's (0.02% BSA / 0.02% Ficoll / 0.02% polyvinylpyrrolidone) 5 mM MgCl2 Hybridization
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