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详询说明书
【产品货号】TLR106RLS-Q【产品说明】PIPETTE TIP 5-300UL LOW RETENTION NATURAL STER 96TIP/INSRT 10INSRT/PK 5PKS/CS【产品简介】5-300 ul 吸头,低吸附处理,无色透明,灭菌,96预装版系统,10插槽/包装,5包装个/箱【品牌】ThermoScientific
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文献和实验Mag-Bind Blood RNA Isolation Protocol (for 50ul Blood)
1. Prepare a Lysis Mixture by mixing one volume of Buffer MRB and one volume of isopropanol. For 96 samples, combine15 ml Buffer MRB with 15 ml isopropanol and mix throughly by vortexing. Add 300 ul Lysis Mixture for each sample at step 3. 2. Add 50 ul
E.Z.N.A. Mag-Bind Blood DNA Protocol (1-100 ul Blood)
. Completely remove and discard the cleared supernatant. 10. Remove the tube containing the Mag-Bind particles from the magnetic separation device. Add 300 ul SPM Buffer to each sample. 11. Resuspend the Mag-Bind particles pellet by vortexing
is cDNA concentration 200 ng/ul too high for qRT-PCR-Rea
but I don't use it so I'm not sure. -soluene- Soluene, When I started this Real Time work about 6 weeks ago, I purified the cDNA first. But I had so little left after this (10-15 ng/ul), that I did not have enough cDNA to make
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