TE缓冲液 Tris-EDTA Buffer (1×TE,

TE缓冲液 Tris-EDTA Buffer (1×TE, pH7.4)

产品简介 

 

TE即Tris-EDTA buffer，也称为T10E1 buffer，是分子生物学的常用缓冲溶液，尤其是涉及到DNA，RNA的过程中保护其不被降解。TE缓冲液由Tris（最常用的pH缓冲液）和EDTA（二价金属离子螯合剂）两种试剂组成，前者常用的pH缓冲范围为7.5-8.5，这种环境下核酸酶的活性较低；后者通过螯合核酸酶所必需的金属离子，从而进一步使核酸酶失活。

 

本品是即用型的1×TE缓冲液，pH7.4，经高压灭菌处理，不含DNase酶，适用于DNA的纯化和保存。也可用作清洗缓冲液（washing buffer）。

产品性质 1×TE工作液含有10 mM Tris-HCl 和1 mM EDTA·Na2，pH7.4

 

注意事项 

 

为了您的安全和健康，请穿实验服并戴一次性手套操作。

 

TE buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA, cDNA or RNA. "TE" is derived from its components: Tris, a common pH buffer, and EDTA, a molecule that chelates cations like Mg2+. The purpose of TE buffer is to solubilize DNA or RNA, while protecting it from degradation.

Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Tris-EDTA (TE) buffer solution, pH 8.0 may also be used as a washing buffer.

 

Short-term storage (weeks) 4 degree centigrade in Tris-EDTA
Medium-term storage (months) -80 degree centigrade in Tris-EDTA
Long-term storage (years) -80 degree centigrade as a precipitate in ethanol
Long-terms storage (decades) -164 degree centigrade or dried.