- ￥1560
- Sino Biological
- 北京
- 69035-A08HL
- 2025年08月12日
- WB
- HEK293 Cells
- Artemisia vulgaris
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
0
- 亚型：
见说明书
- 形态：
液体
- 保存条件：
4℃
- 克隆性：
无
- 标记物：
见说明书
- 适应物种：
Artemisia vulgaris
- 保质期：
12个月
- 抗原来源：
见说明书
- 目录编号：
69035-A08HL
- 级别：
免疫学
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 宿主：
HEK293 Cells
- 应用范围：
WB
- 浓度：
见说明书
- 靶点：
Major pollen allergen Art v 1
- 抗体英文名：
Artemisia vulgaris Major pollen allergen Art v 1 CHO Cell Lysate (WB positive control)
- 抗体名：
Artemisia vulgaris Major pollen allergen Art v 1 CHO Cell Lysate (WB positive control)
- 规格：
300 µg
反应种属：Artemisia vulgaris
裂解液靶点：Major pollen allergen Art v 1
裂解液应用：WB
裂解液保存条件：Store at 4℃. After re-dissolution, aliquot and store at -80℃.
裂解液产品描述：HEK293 Cell Artemisia vulgaris Major pollen allergen Art v 1 transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer).
裂解液表达宿主：HEK293 Cells
裂解液制备方法：Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解液Buffer：Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
裂解液质控信息：12.5% SDS-PAGE Stained with Coomassie Blue after protein purification.
裂解液使用建议：1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃.
裂解液稳定性：Samples are stable for up to twelve months from date of receipt.
裂解液使用说明：Western blot (WB): Use at an assay dependent dilution. Other Applications: Not tested.
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文献和实验for cell lysis: 10 mM Tris-HCl, pH 7.4,50 mM NaCl, 5 mM EDTA, 50 mM NaF, 30 mM Na 4 P 2 O 7 ,and 1% (v/v) Triton-X100. 5. Protease inhibitors and phosphatase inhibitor are added fresheach time with the following final concentrations: 10 μg/mLaprotinin
” 建议：最开始做过湿转（过夜的那种），太费事费时，效果也不如半干转。 蛋白来源：293T细胞 蛋白名称（可保密）： 蛋白分子量：95 KD & 35 KD WB用膜类型、孔径：PVDF 转膜方式（恒压、恒流）：湿转 恒压 90 V-110 V，控制电流不要超过300 mA。 转膜时间：70 min
Overview of Protein Microarrays
, tissue or cell lysates can also be fractionated and spotted on a slide to form a reverse?phase protein microarray. Applications of protein microarrays, especially functional protein microarrays, have flourished over the past decade as the fabrication
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