Tween-20（吐温-20）

Tween-20（吐温-20）

产品简介 

吐温-20（Tween-20），又称聚山梨酯-20（Polysorbate-20），一种来源于山梨聚糖类酯的吐温类非离子型表面活性剂，易混溶于水，广泛应用于生物化学研究中，包括：

1）膜结合蛋白提取实验中，常以2%浓度去除膜周边蛋白；

2）基于膜检测的免疫学实验中，常以0.05%的浓度用作一种封闭剂；

3）还可以0.005~0.5%的浓度用于裂解哺乳动物细胞。

 

产品性质 

中文别名（Chinese Synonym） 聚山梨酯-20
英文别名（English Synonym） 
Polysorbate 20, Polyoxyethylene (20) Sorbitan Monolaurate, TWEEN® 20; Glycosperse L-20X, Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate, 
PEG(20)sorbitan monolaurate
CAS号（CAS NO.） 9005-64-5
分子量（Molecular Weight） ~1228
密度（Density） 1.095 g/mL(Lit.)
外观（Appearance） 透明，黄色至琥珀色黏稠液体
临界胶束浓度（CMC） 0.06 mM (60 mg/L or 6 × 10-3 mole/L)
比重（Specific Gravity） 1.08-1.13
含水量（Water Content） ≤3%
皂化值（saponification number） 40-50
溶解性（Solubility） 易混溶于水（50mg/ml），混溶于醇类，二，乙酸乙酯

 

注意事项 

1）本品不建议高压灭菌，建议用0.22μm滤膜过滤除菌。滤膜除菌前可能需要微热Tween-20到40°C，且用热水替换部分Tween-20。

2）为了您的安全和健康，请穿实验服并戴一次性手套操作。

 

Biotechnical applications

In biological techniques and sciences, polysorbate 20 has a broad range of applications. For example, it is used:

as a washing agent in immunoassays, such as Western blots and ELISAs. It helps to prevent non-specific antibody binding. In this major application, it is dissolved in Tris-Buffered Saline or Phosphate buffered saline at dilutions of 0.05% to 0.5% v/v. These buffers are used for washes between each immunoreaction, to remove unbound immunologicals, and eventually for incubating solutions of immunoreagents (labeled antibodies) to reduce unspecific background.
to saturate binding sites on surfaces (i.e., to coat polystyrene microplates, generally combined with proteins such as BSA).
to stabilize proteins purified protein derivative (PPD) solution used in skin testing for tuberculosis exposure
as a solubilizing agent of membrane proteins
for lysing mammalian cells, at a concentration of 0.05% to 0.5% v/v, generally combined with other detergents, salts and additives.