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- 信裕生物
- XY0352H
- 中国
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 英文名：
Triton X-100
- 保质期：
1年
- 供应商：
上海信裕生物科技有限公司
- 保存条件：
室温保存
- 规格：
100mL;500mL
产品简介 Triton X-100，中文名字曲拉通X-100，一种非离子表面活性剂（结构类似于igepal、NP-40），在分子生物学及免疫学中用于促进脂质和蛋白质的溶解，可用于多种实验，如细胞裂解、蛋白提取、酶类储存液以及其他蛋白稳定液的配制、ICC/IHC等。
产品性质
英文别名（English Synonym） X-100；T-octylphenoxypolyethoxyethanol
CAS号（CAS NO.） 9002-93-1
分子式（Formula） C34H62O11
分子量（Molecular Weight） 647
密度（Density） 1.057g/ml-1.065g/ml（20℃）
外观（Appearance） 无色或淡黄色粘稠夜体。
纯度（Purity） >98%
溶解性（Solubility） 能溶于水、甲苯、二甲苯、乙醇，不溶于
注意事项
为了您的安全和健康，请穿实验服并戴一次性手套操作。
Triton X-100 (C14H22O(C2H4O)n) is a nonionic surfactant that has a hydrophilic polyethylene oxide chain (on average it has 9.5 ethylene oxide units) and an aromatic hydrocarbon lipophilic or hydrophobic group.
Undiluted Triton X-100 is a clear viscous fluid (but less viscous than undiluted glycerol) owing to the hydrogen bonding of its hydrophilic polyethylene oxide parts. Undiluted Triton X-100 has a viscosity of about 270 centipoise at 25 °C which comes down to about 80 centipoise at 50 °C.
Triton X-100 is a commonly used detergent in laboratories. Triton X-100 is widely used to lyse cells to extract protein or organelles, or to permeabilize the membranes of living cells.
Some applications include
Industrial purpose (plating of metal)
Permeabilizing unfixed (or lightly fixed) eukaryotic cell membranes
Solubilizing membrane proteins in their native state in conjunction with zwitterionic detergents such as CHAPS
Part of the lysis buffer (usually in a 5% solution in alkaline lysis buffer) in DNA extraction
Reducing surface tension of aqueous solutions during immunostaining (usually at a concentration of 0.1-0.5% in TBS or PBS Buffer)
Dispersion of carbon materials for soft composite materials
Restricting colony expansion in Aspergillus nidulans in microbiology
Decellularization of animal-derived tissues
Removing SDS from SDS-PAGE gels prior to renaturing the proteins within the gel
Disruption of cell monolayers as a positive control for TEER measurements.
Apart from laboratory use, Triton X-100 can be found in several types of cleaning compounds, ranging from heavy-duty industrial products to gentle detergents. It is also a popular ingredient in homemade vinyl record cleaning fluids together with distilled water and isopropyl alcohol.[citation needed] It is a good micellar catalyst.
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文献和实验Triton X-100是一种非离子型去垢剂 分子式 ：C14 H22 O(C2 H4 O)n 用途 ：Triton X-100是一种比较温和的去垢剂（表面活性剂或称界面活性剂），常作为添加剂使蛋白保持稳定，尤其是膜蛋白。 性质 ： 1. Triton X-100对细菌等微生物没有杀伤
Triton X-114 Phase Partitioning for Antigen Characterization
In 1981, Bordier (1 ) first demonstrated that Triton X-114 could be exploited as a means of separating hydrophilic and integral membrane proteins. Since then, Triton X-114 phase partitioning has been extensively used for identification
的帖子，共：1个★ Triton X-100 曲拉通X-100的英文全称 ★以上为检索SOS版块发表的帖子，共：1个★ （交流）介绍6种针对免疫荧光染色的细胞固定方法 （请教）几种液体的配法 （求助）：免疫组化问题 (求助)Triton X-100的使用方法 （求助）Triton溶液配方 （求助）关于Triton的浓度和使用 （求助）关于免疫组化的洗涤液 （求助）请高手帮助翻译一下 （求助）细胞免疫组化破膜问题 Re:（交流）免疫组化染色过程中存在的问题、原因
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