曲拉通 (Triton X-100)

曲拉通 (Triton X-100)

产品简介 Triton X-100，中文名字曲拉通X-100，一种非离子表面活性剂（结构类似于igepal、NP-40），在分子生物学及免疫学中用于促进脂质和蛋白质的溶解，可用于多种实验，如细胞裂解、蛋白提取、酶类储存液以及其他蛋白稳定液的配制、ICC/IHC等。

 

产品性质 

英文别名（English Synonym） X-100；T-octylphenoxypolyethoxyethanol
CAS号（CAS NO.） 9002-93-1
分子式（Formula） C34H62O11
分子量（Molecular Weight） 647
密度（Density） 1.057g/ml-1.065g/ml（20℃）
外观（Appearance） 无色或淡黄色粘稠夜体。
纯度（Purity） >98%
溶解性（Solubility） 能溶于水、甲苯、二甲苯、乙醇，不溶于

 

注意事项 

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Triton X-100 (C14H22O(C2H4O)n) is a nonionic surfactant that has a hydrophilic polyethylene oxide chain (on average it has 9.5 ethylene oxide units) and an aromatic hydrocarbon lipophilic or hydrophobic group.

Undiluted Triton X-100 is a clear viscous fluid (but less viscous than undiluted glycerol) owing to the hydrogen bonding of its hydrophilic polyethylene oxide parts. Undiluted Triton X-100 has a viscosity of about 270 centipoise at 25 °C which comes down to about 80 centipoise at 50 °C.

Triton X-100 is a commonly used detergent in laboratories. Triton X-100 is widely used to lyse cells to extract protein or organelles, or to permeabilize the membranes of living cells.

Some applications include

Industrial purpose (plating of metal)
Permeabilizing unfixed (or lightly fixed) eukaryotic cell membranes
Solubilizing membrane proteins in their native state in conjunction with zwitterionic detergents such as CHAPS
Part of the lysis buffer (usually in a 5% solution in alkaline lysis buffer) in DNA extraction
Reducing surface tension of aqueous solutions during immunostaining (usually at a concentration of 0.1-0.5% in TBS or PBS Buffer)
Dispersion of carbon materials for soft composite materials
Restricting colony expansion in Aspergillus nidulans in microbiology
Decellularization of animal-derived tissues
Removing SDS from SDS-PAGE gels prior to renaturing the proteins within the gel
Disruption of cell monolayers as a positive control for TEER measurements.
Apart from laboratory use, Triton X-100 can be found in several types of cleaning compounds, ranging from heavy-duty industrial products to gentle detergents. It is also a popular ingredient in homemade vinyl record cleaning fluids together with distilled water and isopropyl alcohol.[citation needed] It is a good micellar catalyst.