印度墨汁 印度墨水 Indian Ink, Biotech

印度墨汁 印度墨水 Indian Ink, Biotech Grade for Biological Stains

产品简介

墨汁荚膜染色对于观察新型隐球菌有很好的作用，印度墨汁(India Ink)属于非常优质的墨汁，新型隐球菌菌体周围存在粘多糖荚膜物，用印度墨汁染色法能证明荚膜的存在。染色后新型隐球菌的荚膜取代了墨汁中的胶状碳粒，菌体被清楚无色透明的晕圈环所绕着。

级别：BR

生物染色：合格

性状：黑色液体

用途：生化研究。生物染色剂。

保存：RT,有效期12个月；

自备材料：离心机；载玻片、盖玻片

 

操作步骤(仅供参考)：

1、样本离心，取沉淀涂于载玻片上，在样本涂片处滴加India Ink。

2、加盖盖玻片，并防止气泡产生。用手指轻轻压一下，使样本与India Ink混合变薄。

3、在低倍镜下寻找有荚膜的细菌，并转高倍镜或油镜下仔细辨认。

 

染色结果

新型隐球菌>>宽厚透亮的荚膜，细胞壁明显
背景>>黑色

 

注意事项

1、已开封India Ink 应在6个月内用完，每次用后尽量密封好，以免挥发或变质。

2、India Ink存储时间较长后易产生沉淀，如果镜下发现颗粒粗糙者应过滤后使用或弃用。

3、目的样本尽量新鲜。

4、样本的纯度和浓度应适宜，浓度过低时在镜下很难寻找到新型隐球菌。

5、样本和印度墨汁分布应均匀。

 

Basic India ink is composed of a variety of fine soot, known as lampblack, combined with water to form a liquid.

 

Microbiologists use India ink to stain a slide containing micro-organisms. The background is stained while the organisms remain clear. This is called a negative stain. India ink, along with other stains, can be used to determine if a cell has a gelatinous capsule. A common application of this procedure in the clinical microbiology laboratory is to confirm the morphology of the encapsulated yeast Cryptococcus spp. which cause cryptococcal meningitis.

Medical researchers use India ink to visualize blood vessels when viewed under a microscope.

 

Scientists performing Western blotting may use India ink to visualized proteins separated by electrophoresis and transferred to a nitrocellulose or PVDF membrane.

Zoological museum specimens were often tagged in India ink, either directly or on a piece of tracing paper stored along the specimen, because of its durability even when submerged in preservative fluids.