- 询价
- EnzyValley
- E603
- 广州
- 2025年12月22日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 供应商：
广州英赞生物科技有限公司
- 库存：
充足
- 英文名：
Advanted Directional RNA Lib Prep Kit
- 保质期：
2年
- 保存条件：
-20℃保存
定向RNA建库试剂盒
产品概述
产品内容
| Components | E603A (24 rxn) | E603B (96 rxn) |
| ● RT Buffer | 144 μL | 576 μL |
| ● RT Enzymes | 48 μL | 192 μL |
| ● Stranded Reagent | 192 μL | 768 μL |
| ● 2ndSS Buffer | 192 μL | 768 μL |
| ● 2ndSS Enzymes | 96 μL | 384 μL |
| ● EP Buffer | 144 μL | 576 μL |
| ● EP Enzyme Mix | 96 μL | 384 μL |
| ● Adapter (for Illumina) | 60 μL | 240 μL |
| ● ED Buffer | 240 μL | 960 μL |
| ● Ligation Master Mix | 720 μL | 960 μL (x 3) |
| ● IPM (Indexed Primer Mix) | 20 μL (x 12) [1] | 10 μL (x 96) [2] |
| ● PfuMax PCR Mix | 600 μL | 1.2 mL (x 2) |
| ● UCS | 72 μL | 288 μL |
[1] RL1020-02内含12个Index，包括IPM 01 ~ 12。
[2] RL1020-03内含96个Index，包括IPM 01 ~ 96。
应用范围
基因表达量分析，发现SNV，可变剪接与融合基因转录本分析
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文献和实验will have a 28s/18s ratio = 2. The "concentration" result may be equated to the amount of RNA (ng) that was submitted. For more details on the interpretation of BiaAnalyzer tracings see the section of the BioAnalyzer Nano 6000 kit manual that discusses assay
by Elbashir et al. (1) that siRNAs with 3' overhanging UU dinucleotides are the most effective. This is also compatible with using RNA pol III to transcribe hairpin siRNAs because RNA pol III terminates transcription at 4-6 nucleotide poly(T) tracts creating
al. (1) that siRNAs with 3' overhanging UU dinucleotides are the most effective. This is also compatible with using RNA pol III to transcribe hairpin siRNAs because RNA pol III terminates transcription at 4-6 nucleotide poly(T) tracts creating RNA
