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文献和实验Purification of KHC Motor Domain Protein
FPLC system 1. Induce cells and harvest as described in Bacterial Expression of Motors . Wash induced cells with HEM + 25 mM NaCl + 0.5 mM PMSF and freeze in 30 mL Oak Ridge centrifuge tubes at -80°C until use
Evaluating Modulators of “Regulator of G‐protein Signaling” (RGS) Proteins
?protein Signaling? (RGS) proteins constitute a class of intracellular signaling regulators that accelerate GTP hydrolysis by heterotrimeric G? subunits. In recent years, RGS proteins have emerged as potential drug targets for modulation by small molecules
phosphate (pH 5.4) for 2 hours at 1-1.5 ml/min. Load the pooled fractions onto the gel-filtration column (load ~ 5 ml per run). This should desalt and buffer-exchange the CD2 protein as well as removing the larger contaminants. 7) Analyse
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