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文献和实验Agarose Gel Electrophoresis of DNA
Agarose Gel Electrophoresis of DNA 1) Dissolve 1 g of agarose in 100 ml of 1X TAE or TBE buffer (gives a 1% gel). See note for making LMP agarose
Co-Expression of human DNA primase p49-p58 subunits
room.Try to get every last bead.Equilibrate & pack column with more wash buffer.If beads are grayish,it's a good sign cause it means you've got a lot of protein retained on them. 12.Elute protein with ice cold elution buffer (~8 ml).Collect 1.0 ml
for sample application. 2. Sample Application and Fractionation: Load the sample usinga Superloop TM at a flow rate of 1 to 2.5 mL/min. If the sampleexceeds the maximum volume that can be efficiently fractionatedon this column (consult the vendor’s
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