In Vitro: BN201 (10 μM; 0.5-4 hours; Hela cells) treatment induces the phosphorylation of NDGR1 in Hela cells in a time-dependent manner. BN201 promotes the survival of cultured neural cells when subjected to oxidative stress or when deprived of trophic factors.
In Vivo: BN201 (12.5-150 mg/kg; intraperitoneal injection; daily; for 30 days; female C57BL/6 mice) treatment displays less axonal loss and neuron loss in both the spinal cord and in the optic nerve than the animals that received placebo.