中文名称: 产黑素普雷沃菌、杀虫贪铜菌 NCTC9336标准菌株基本信息 保藏机构:NCTC 菌种名称:NCTC9336 , NCTC 9336 英文名称:Prevotella melaninogenica 中文名称:产黑素普雷沃菌、杀虫贪铜菌 存储人:(Oliver and Wherry) Shah and Collins 来源历史:<< NCTC << Sharpe << L. P. Garrod, St. Bartholomew's Hospital, London 其他保藏中心编号:NCTC 9336 分离来源:文森特牙龈炎 菌种特性 备注: 培养条件:37°C 生物安全等级:2 氧气需求:需氧 培养基: CHOPPED MEAT MEDIUM Ground beef (fat free) 500.0 g 1N NaOH 25.0 mL Distilled water 1.0 L Peptone 30.0 g Yeast extract 5.0 g L-cysteine·HCl 0.5 g K2HPO4 5.0 g 0.025% Resazurin solution 4.0 ml Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix meat, water and NaOH and bring to a boil while stirring. Cool to room temperature, skim fat off surface, filter and retaining both meat particles and filtrate. Add sufficient distilled water to filtrate to restore 1.0 L original volume. To the filtrate add peptone, yeast extract, K2HPO4 and resazurin solution. Boil, then cool under O2-free nitrogen and 3% hydrogen, and add 0.5 g L-cysteine·HCl. Adjust pH to 7.0. Dispense 4-5 parts fluid to 1 part meat particles per test tube. Cap with rubber stoppers under N2 and H2 and autoclave in press for 15 minutes under fast exhaust.