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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8°C
- 克隆性:
单克隆
- 适应物种:
Human, Mouse, Rat.
- 库存:
大量
- 供应商:
默克生命科学
- 宿主:
rabbit
- 应用范围:
Immunohistochemistry (Paraffin);Western Blotting;Immunocytochemistry
- 靶点:
Calretinin
- 抗体英文名:
ANTI-CALRETININ, CLONE 3K22, ZOOMAB RAB
- 规格:
25UL
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文献和实验Immunostaining with Anti-Digoxigenin Alkaline Phosphatase
Materials Anti-digoxigenin-AP conjugate (Roche #11093274910, 150U/200 uL, $168) Levamisole (Sigma #T1512, 2 g, $10) (blocks endogenous phosphatase activity) BCIP (Roche #11383221001, 3 mL, $49) NBT (Roche #11383213001, 3 mL, $48)
PREPARATION OF ANTI-BLEACHING MOUNTING MEDIUM
PREPARATION OF ANTI-BLEACHING MOUNTING MEDIUM Materials 1. p-phenylenediamine, 1%(w/v) in distilled water, 1 ml. Adjust pH to 9.5 with NaOH. Use the highest grade from Sigma. 2. glycerol. 3. 10x simple PBS. Procedure
in a humid chamber for 1 hour, RT. Blot off antibody, wash 2 times 5 min. each in 1x PBS. Prepare the working dilution of the secondary antibody (Horse anti-mouse IgG-Vector Catalog No. BA2001) in 1.0% BSA/PBS, 2.0% normal horse serum. Prepare
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