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- 详细信息
- 文献和实验
- 技术资料
- 规格:
>20次
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| AC033 | Caspase-3(激活型)抗体(兔单抗) | >20次 | 1013.00元 |
| 来源 | 用途 | 交叉反应性 | 抗体识别位点 | Caspase-3分子量 |
| Rabbit | WB, IP, IF, IHC, F | H, M, R | Cleaved Caspase-3 at Asp175 | 19/17 kD |
WB,Westernblot;IP,Immunoprecipitation;IF,Immunofluorescence;IHC,Immunohistochemistry;F,Flow cytometry.
H,human;M,mouse;R,Rat.
本Caspase-3(激活型)抗体为Cleaved Caspase-3(Asp175)抗体,为进口分装,用人工合成的一段邻近Asp175的human Caspase-3的多肽片段,并通过适当修饰后免疫兔子制备而成的抗Caspase-3(cleaved at Asp175)兔单克隆抗体。克隆号为5A1(Clone 5A1),是一种应用较广的检测Cleaved Caspase-3(Asp175)抗体。
本Caspase-3(激活型)抗体仅仅识别在Asp175位剪切后产生的19和17kDCaspase-3片段,即所谓的激活型Caspase-3。不识别全长的Caspase-3(32kD),也不识别Caspase-3在其它位点被剪切产生的Caspase-3片段。
本抗体可以通过免疫荧光或免疫组化检测细胞凋亡过程中Caspase-3的激活。本抗体染色呈阳性的细胞可以判定为Caspase-3被激活的细胞。
Caspase-3是细胞凋亡过程中最关键的执行分子(key executioner)之一,可以剪切细胞凋亡过程中的许多关键蛋白,例如PARP。Caspase-3的激活需要从没有活性的全长Caspase-3(35kD),在Asp28和Ser29之间及Asp175和Ser176之间进行剪切,产生有活性的17kD亚基和12kD的亚基。
Caspase-3的激活常被作为细胞凋亡的一个重要指标。
配套提供了Western一抗稀释液,可以用于Western检测时的一抗稀释。
建议抗体使用时的稀释比例为(实际使用时需根据抗原水平的高低作适当调整):
| WB | IP | IF | IHC | F |
| 1:1000 | 1:100 | 1:500 | 1:100 | 1:100 |
本抗体如果用于常规的Western检测至少可以检测20次。
包装清单:
| 产品编号 | 产品名称 | 包装 |
| AC033-1 | Caspase-3(激活型)抗体(兔单抗) | 20μl |
| AC033-2 | Western一抗稀释液 | 20ml |
| — | 说明书 | 1份 |
保存条件:
Caspase-3(激活型)抗体-20℃保存,Western一抗稀释液-20℃或4℃保存,一年有效。Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
对于本抗体,Western检测时一抗要4℃缓慢摇动过夜,如果仅短时间与一抗孵育检测效果较差。
在Western实验后,请注意回收稀释的抗体。回收的抗体在进行Western实验时至少可以重复使用10次。稀释后的抗体,包括已经使用过的稀释抗体,4℃保存。
回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
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【求助】caspase-3抗体与激活型caspase-3抗体
环佩从容 要做下一步实验啦,这两个抗体选哪个呀,目前研究有没有区分哪一个更好点啊 ronaldo_zj 楼主您好。 1. 楼主需要知道Pro caspase-3以及Cleaved caspase-3的功能以及检测手段,不是说哪个好那个不好的问题,而是根据你的课题需要检测何种片段的问题。请楼主先具体查阅关于caspase活化的文献后再定好实验计划; 2. 目前多数研究均认为Cleaved
【求助】请问western blot做凋亡检测时,识别caspase-3酶原和激活的caspase-3的抗体是相同的吗?
lsdcfhyj 以及检测PARP的裂解前后蛋白水平用的抗体是否都是一样的? edwardellen 有两者都识别的,也有专门识别剪切体不识别酶原的,用过cell signal的,两种都很好用。 PARP也有可以同时识别全长和剪切体的抗体。 magichunter 检测caspase-3和激活型的caspase-3分别需要两种不同的抗体,一般CST公司卖的分别包括:cleaved
,鼠单抗已从单纯的生物实验室科研及临床诊断发展到治疗性抗体药物。目前的抗体药物大多来源于小鼠或噬菌体展示库。2019 年 3 月,首个骆驼源纳米抗体 Cablivi 获得 FDA 批准上市。2019 年 10 月,迎来首个兔单抗药物 Beovu,是抗体药物发展历程中的一个里程碑。临床使用的大多数治疗用抗体均需进行体外亲和力成熟,以提高抗体的亲和力。这一过程耗时长(8-12 个月),最终亲和力在 10-9mol/L。而兔单抗不需经体外亲和力成熟,就能达到 10-11 至 10-13mol/L。另外,兔











