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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Mouse BM10-37 cytotoxic T cells
- 亚型:
IgG2b
- 形态:
液体
- 保存条件:
4°C避光无菌保存母液。若需保存超过6个月, 分装储存在-20°C无霜冰箱,避免反复冻融
- 适应物种:
mouse
- 保质期:
按照要求储存,收到产品后一年
- 级别:
临床前级别
- 库存:
1 vial
- 供应商:
上海优宁维
- 宿主:
hamster
- 应用范围:
in vivo T cell depletion
- 浓度:
批次间差异,一般介于2-10 mg/ml
- 靶点:
CD3ε F(ab')2 fragment
- 抗体英文名:
anti-mouse CD3ε F(ab')2 fragment
- 抗体名:
anti-mouse CD3ε F(ab')2 fragment
- 规格:
10 mg
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文献和实验T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
Immunostaining with Anti-Digoxigenin Alkaline Phosphatase
cut paraffin sections and deparaffinize with xylene and graded EtOH into PBS. Block slides with 1/20x serum/PBS (use the appropriate blocking serum, i.e. same as 1º Ab), 10'. Wash in PBS 2' x3. Add digoxigenin labeled 1º antiobody.
Protocol for anti-HA antibody Western Blotting
of interest. 2) Transfer proteins in acrylamide gel to nylon membrane. 3) Block overnight @4℃ in PBS (or TBS, it doesn't matter) with 5% non-fat dry milk. I dilute 2.5 mg in 50 ml. 4) Wash off excess milk sol'n with PBS x3 .(important if you are re-using your antibody
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