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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C
- 英文名:
SbfI
- 库存:
100
- 供应商:
ABclonal
- 规格:
500 U/2500 U
| 规格: | 500 U | 产品价格: | ¥530.0 |
|---|---|---|---|
| 规格: | 2500 U | 产品价格: | ¥2300.0 |
产品描述
5'…CCTGCA/GG...3'3'…GG/ACGTCC…5'
产品组分
| SbfI (10,000 U/mL) | RM21629 |
| 10X Buffer CutS | RM20103 |
产品信息
| 应用 | Restriction Enzyme Digestion | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 活性定义 | One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μL. | ||||||||
| 保存条件 | 10 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 200 μg/mL BSA, 50% Glycerol, pH 7.4 @ 25°C | ||||||||
| 保存温度 | -20°C | ||||||||
| 反应条件 | 1X Buffer CutS, Incubate at 37°C . | ||||||||
| 反应缓冲液或预混液 | 1X Buffer CutS: 50 mM Potassium Acetate, 20 mM Tris-acetate, 10 mM Magnesium Acetate, 100 μg/ml BSA, pH 7.9 @ 25°C |
||||||||
| 是否快切 | 是 (在推荐反应体系下5-15 min 完全酶切底物) | ||||||||
| 热失活 | 80°C for 20 min | ||||||||
| 甲基化敏感性 |
|
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| ABclonal反应缓冲液中的活性 |
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文献和实验Measurements of Cytosolic Ion Concentrations in Live Cells
Here, we describe a series of methods suitable for the measurement of cytosolic ion concentrations in living plant cells using ion selective dyes. We describe procedures for the use of SBFI for the measurement of Na+ in live cells
G/CGCGC SbfI CCTGCA/GG BssSI CACGAG,-5,-1 ScaI AGT/ACT BstBI TT/CGAA SciI CTC/GAG BstEII G/GTNACC SelI /CGCG BstUI CG/CG SexAI A/CCWGGT BstXI CCANNNNN/NTGG SfiI GGC***NN/NGGCC BstZ17I GTA/TAC SfoI GGC/GCC Bsu36I CC/TNAGG SgrAI CR/CCGGYG ClaI
Measurement of Cation Movement in Primary Cultures Using Fluorescent Dyes
) 1 mM SBFI acetoxymethyl ester (SBFI AM; Molecular Probes) in dimethyl sulfoxide (DMSO) BSA Primary
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