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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
1年
- 库存:
大量
- 供应商:
无锡莱弗思生物实验器材有限公司
- 规格:
10 µg
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文献和实验Chromatin immunoprecipitation: optimization, quantitative analysis and data normalization
control) relative to the level of the signal of interest (ChIP sample). This hampers distinguishing a ''true signal'' from the background signal. Therefore, we recommend setting up ChIP using an antibody against an invariant domain of histone H3 [19,20
from the antibody-bound and antibody-unbound fractions (from Chromatin Immunoprecipitation on Unfixed Chromatin from Cells and Tissues to Analyze Histone Modifications). Control genomic DNA should be used as well. For each PCR reaction, we use 20-50 ng of template
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