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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
1年
- 库存:
大量
- 供应商:
无锡莱弗思生物实验器材有限公司
- 规格:
100 µg
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文献和实验Affi nity Purifi cation of MLL3/MLL4 Histone H3K4 Methyltransferase Complex
Methylation on histone H3 lysine 4 (H3K4) correlates with actively transcribed genes. In mammalian cells, there exist multiple Set1-like histone H3K4 methyltransferase complexes, which have overlapping but distinct subunit compositions
PROTOCOL To 1.5 mL eppendorf tubes add: 200 µg of protein extract (see Western blot protocol for protein sample preps) q.s. to 300 µL with RIPA (with protease and phosphatase inhibitors). Primary antibody (amount determined
Evaluation of Histone-Modifying Enzymes in Stem Cell Populations
The histone demethylases are a relatively novel family of histone-modifying enzymes. Their gene expression suggests that each of the subfamily members may have a discrete role in cell function. The KDM5 family of H3K4 histone demethylases
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