Universal Protease Activity EL

The Eagle Biosciences Universal Protease Activity ELISA Assay Kit measures activity of proteases like matrix metalloproteases (MMPs), A Disintegrin And Metalloproteinase with Thrombospondin Motifs (ADAMTS), especially High temperature requirement factor A (HtrA). It consists of two modules: Protease module and the ELISA module. In this assay, a biotinylated ß-Casein coupled beads is first digested with protease(s)/HtrA1. The proteolytic cleavage of the substrate releases a biotinylated ß-Casein fragment, without beads, that will bind on streptavidin-coated plate and can be further quantified with one specific monoclonal antiß-Casein antibodies and with a Peroxidase-labeled antibody The Universal Protease Activity ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.