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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
4℃
- 保质期:
1-2 years
- 英文名:
Total FGF-21 ELISA Assay
- 库存:
999
- 供应商:
Eagle Biosciences Inc.
- 规格:
96T or More
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文献和实验is separated from 14C Cm by phase extraction. ASSAY Make up cocktail, keep on ice; does 10 assays 0.25 M Tris-Cl pH 8.0 0.7 ml (0.07/assay) 14C Cm (57µCi/µmol; 25µCi/ml) 0.1 ml (10µl/assay) Bu CoA (5mg/ml) 0.2 ml (20µM/assay) Total 1.0 ml (100µl/assay
down for 1 min. 20. Remove 400 µl into scintillation tubes. 21. Add 10 ml of scintillation liquid. 22. Count it for 2 min. (total cpm's in reaction is 210000-230000)
CAT ASSAY[TLC METHOD] 1. Transfer cells to a 15 ml tube. 2. Add 5 ml TBS
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