S100A8/A9 (MRP8/14, Calprotect
in, Mouse/Rat) ELISA Assay Kit (Eagle Biosciences, CAT# KR6936)- 询价
- Eagle Biosciences
- 美国
- KR6936
- 2025年07月16日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
4℃
- 保质期：
1-2 years
- 英文名：
S100A8/A9 (MRP8/14, Calprotectin, Mouse/Rat) ELISA Assay Kit
- 库存：
999
- 供应商：
Eagle Biosciences Inc.
- 规格：
96T or More
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
ELISA (Enzyme-Linked ImmunoadSorbent Assay)
H2O can also be used -> add 1.42g for 1x and 14.2g for 10x PBS. Make up to 1 litre with dd H2O. PBS wash: 100ml 1x PBS 10ul Tween (0.01%, final concentration) Citrate/Acetate Buffer pH6.0: 1. 500ml 0.1M Sodium Acetate Sodium acetate - 4.1g. dd H2O - 500ml
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
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