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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
4℃
- 保质期:
1-2 years
- 英文名:
MutaGEL MTHFR PCR Assay
- 库存:
999
- 供应商:
Eagle Biosciences Inc.
- 规格:
96T or More
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文献和实验Tailed RT-PCR for the Quantitation of Chloramphenicol Acetyl Transferase (CAT)mRNA
. Subsequently, CAT activity is measured as a readout by thin-layer chromatography (TLC) or the levels of CAT protein are determined using an enzyme-linked immunosorbent assay (ELISA). However, most transfections-whether stable or transient-result in low levels
鉴定。彩色PCR(Color complement assay)直译为“着色互补性检测”,是LP-PCR的一种,彩色PCR意译更为明确:它用荧光染料标记引物的5’端。荧光染料JOE和FAM呈绿色荧光;TAMRA呈红色荧光;COUM呈蓝色荧光。不同荧光标记的引物同时参加反应,扩增后的目的基因会分别带有引物5’端的染料,通过电泳或离心沉淀,肉眼就可以根据不同荧光的色泽判断目标基因是否存在及扩增基因的类型。通常仅需2种不同颜色的引物,一种作为基因检测引物;另一种作为控制条件的内对照,即可诊断基因
Bartonella henselae is the causative agent of cat-scratch disease (CSD), usually presenting itself as a �self-limiting lymphadenopathy. In this chapter an internally controlled Taqman probe-based real-time PCR targeting the groEL gene
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