50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications
WB: 1 : 1000 up to 1 : 10000 (AP staining) suitable for Dot Blot IP: yes (see remarks) ICC: not tested yet IHC: not tested yet IHC-P/FFPE: not tested yet ELISA: yes suitable for sandwich-ELISA
Immunogen
N6-methyladenosine fused to BSA.
Reactivity
Reacts with: human, rat, mouse, eukaryotes, prokaryotes. Other species not tested yet.
Specificity
Specific for N6-methyladenosine (m6A).
Remarks
IP: Extracts from eukaryotic and prokaryotic cells. Standard protocol for the IP of nuclear extracts is provided with the product. For the isolation of m6A modified RNA from total or mRNA preparations the protocol according to et al. (2013) is recommended.