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Alexa Fluor® 488-AffiniPure Do

nkey Anti-Human IgG, Fcγ Fragment Specific (min X Bov,Hrs,Ms Sr Prot)
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  • Jackson ImmunoResearch已认证
  • US
  • 709-545-098
  • 2026年02月28日
  • Donkey
  • Human
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  • 企业认证

    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

    • 亚型

      IgG, Fcγ fragment

    • 形态

      Freeze-dried solid

    • 保存条件

      Store freeze-dried solid at 2-8°C.

    • 克隆性

      单克隆

    • 标记物

      Alexa Fluor® 488

    • 适应物种

      Human

    • 保质期

      One year from date of rehydration.

    • 抗原来源

      IgG, Fcγ fragment

    • 供应商

      Jackson ImmunoResearch Laboratories Inc.

    • 宿主

      Donkey

    • 抗体英文名

      Alexa Fluor® 488-AffiniPure Donkey Anti-Human IgG, Fcγ Fragment Specific (min X Bov,Hrs,Ms Sr Prot)

    • 抗体名

      AffiniPure Whole IgG

    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc portion of human IgG heavy chain but not with the Fab portion of human IgG. No antibody was detected against human IgM or IgA, or against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, horse and mouse serum proteins, but it may cross-react with immunoglobulins from other species.

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    图标文献和实验
    相关实验
    • 激光共聚焦实验服务案例介绍

      多余液体;加A蛋白一抗50μL(1:100),4℃下过夜;PBS洗3次,每次5min,加荧光标记的A蛋白二抗 [Alexa Fluor® 488 donkey anti-goat IgG (H+L)] 50μL(1:200),室温下放置60min;PBS冲洗3次,加B蛋白一抗50μL(1:500)37℃下放置1h,PBS洗3次,每次5min;加荧光标记的B蛋白二抗[(Anti-Rabbit IgG (H+L), F(ab')2 Fragment)] 50μL(1:200),置于室温下 60 min

    • 流式细胞方案

      细胞仪检测。或者继续 E1 步 DNA 染色。 E. DNA 染色(可选) 1. 将细胞重悬在 0.5 ml DNA 染色液中(例如 DRAQ5® #4084)。 2. 室温至少孵育 5 分钟。 3. 流式细胞仪进行分析。 ☀ 推荐的二抗: 抗兔:  Anti-Rabbit IgG(H+L), F(ab')2 FragmentAlexa Fluor® 488 Conjugate)#4412  Anti-Rabbit IgG(H+L), F(ab

    • Making Boiled Donkey (or whatever) Serum (BDS) for Blocking

      aliquot, but maybe if it's a  fresh aliquot use it at 1:400) for 30 minutes at 37oC. 11) Rinse 3 times for 5 minutes each rinse in PHEM-T. 12) Put on secondary (I use Donkey anti-mouse-Rhodamine Red X) at 1:100 in block for 30 minutes at 37oC. 13

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    Alexa Fluor® 488-AffiniPure Donkey Anti-Human IgG, Fcγ Fragment Specific (min X Bov,Hrs,Ms Sr Prot)
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