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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
- 亚型:
IgG (H+L)
- 形态:
Freeze-dried solid
- 保存条件:
Store freeze-dried solid at 2-8°C.
- 克隆性:
单克隆
- 标记物:
Rhodamine (TRITC)
- 适应物种:
Goat
- 保质期:
One year from date of rehydration.
- 抗原来源:
IgG (H+L)
- 供应商:
Jackson ImmunoResearch Laboratories Inc.
- 宿主:
Rabbit
- 抗体英文名:
Rhodamine (TRITC)-AffiniPure Rabbit Anti-Goat IgG (H+L) (min X Hu Sr Prot)
- 抗体名:
AffiniPure Whole IgG
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文献和实验E L I S A w i t h p l a t e l e t s
prepare platelets from control and immunized mice distribute 2.5 x106 platelets per well on F-bottomed MaxiSorp Nunc-Immuno microplates (GibcoBRL, Roskilde, Denmark), centrifuge at 3000 rpm, 7 min, 10ѓC. fix with PBS-PF
Anti-GFP/BrdU In Situ Immunostaining
by adding 1 mL PBS, 3' each. Add 0.3 mL rabbit anti-GFP (1:200 v/v, plus 1% goat serum in PBS) and incubate 1 hr. at room temp. Wash 3x in PBS. Post-fix in 1 mL 4% paraformaldehyde x15', room temp. Wash 3x in PBS. Denature DNA with 1 mL
-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
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