The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
亚型:
IgG (H+L)
形态:
Freeze-dried solid
保存条件:
Store freeze-dried solid at 2-8°C.
克隆性:
单克隆
标记物:
Coumarin AMCA
适应物种:
Rat
保质期:
One year from date of rehydration.
抗原来源:
IgG (H+L)
供应商:
Jackson ImmunoResearch Laboratories Inc.
宿主:
Goat
抗体英文名:
AMCA-AffiniPure F(ab')2 Fragment Goat Anti-Rat IgG (H+L) (min X Hu,Bov,Hrs Sr Prot)
抗体名:
AffiniPure F(ab')2 Fragment
F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G.Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rat IgG. It also reacts with the light chains of other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine and horse serum proteins, but it may cross-react with immunoglobulins from other species.