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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at 2-8°C under sterile conditions.
- 保质期:
One year from date of receipt.
- 英文名:
ChromPure Rat Transferrin
- 供应商:
Jackson ImmunoResearch Laboratories Inc.
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文献和实验(here we describe the preparation of cytosol from adult rat brains, Steps 1�8) and fluorescently labeled endosomes are needed (we label early endosomes by incubating cultured cells with externally added fluorescent cargo molecules by endocytotic uptake, Steps 9�24). After labeling
by centrifugation. Cell culture. 1. For tissue culture, the collected cells were dispersed with DMEM/Ham F12 containing 10% FCS, 5% chicken embryo extract, and 5% rat serum, as well as 2 mM glutamine, penicillin, and streptomycin. 2.
Purification of Endotoxin Free mAbs
is applicable to most rat and mouse IgG antibodies (not IgM), although individual monoclonal antibodies may require modification of either the pH or ionic strength for optimal purification. Cation exchange using Fast Flow S-Sepharose (Sigma S 1264) at pH
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