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- 详细信息
- 文献和实验
- 技术资料
- 库存：
现货
- 英文名：
Monensin Solution (1,000X)
- 供应商：
上海善然生物科技有限公司
- 保存条件：
低温
- 规格：
1 mL
Monensin Solution (1,000X)
Catalog# / Size 420701 / 1 mL
Description Monensin is a protein transport inhibitor commonly used to enhance intracellular cytokine
staining signals by blocking transport processes during cell activation. Especially useful for the
intracellular staining of cytokines, monensin leads to the accumulation of most cytokines at
the Golgi Complex/Endoplasmic Reticulum (see Jung, et al., 1993). Optimal conditions for
use are cell type and time-dependent. Typically, protein transport inhibitors are included
during in vitro cell activation cultures for 4-24 hours prior to harvest (see references below for
additional information). Monensin Solution is supplied as a 1,000X solution, which should be
diluted to 1X in cell culture medium.
Product Details
Formulation Monensin Solution is supplied as a 1000X working solution in 70% Ethanol. Dilute to 1X in cell
culture medium.
Concentration 2.0 mM
Storage & Handling Store the solution between 2°C and 8°C. Monensin is known to be toxic; avoid direct body contact.
Ethanol is flammable; keep away from sources of fire.
Application ICFC - Quality tested
Recommended Usage Dilute the 1000X solution to 1X in the tissue culture medium. It is recommended that cells are
cultured with monensin for ≤ 24 hours, as this can become toxic for cell viability.
Application References
(PubMed link indicates
BioLegend citation)
1. Smeltz RB. 2007. J. Immunol. 178:4786.
2. Fuse S, et al. 2007. J. Immunol. 178:5227.
3. Durkin ET,et al. 2008. Blood 10:1182.PubMed
4. Durkin ET, et al. 2008. Blood 112:5245. PubMed
5. Liu XS, et al. 2009. J. Immunol. 183:51. PubMed
6. Mattarollo SR, et al. 2010. J. Immunol. 184:1242. PubMed
7. Yang M, et al. 2010. J. Immuonl. 184:3321. PubMed
8. Mattarollo SR, et al. 2010. J. Immunol. 184:5663. PubMed
9. Wood MW, et al. 2011. Infect Immun. 79:708. PubMed
10. Janune D, et al. 2011. FEBS Lett. 585:3033. PubMed
11. Fiorenza S, et al. 2012. J. Immunol. 189:5622. PubMed
12. Asgari E, et al. 2013. Blood. 122:3473. PubMed
Product Citations
1. Durkin E, et al. 2008. Blood. 112:5245. PubMed
2. Liu X, et al. 2009. J Immunol. 183:51:00. PubMed
3. Mattarollo S, et al. 2010. J Immunol. 184:5663. PubMed
4. Yang M, et al. 2010. J Immunol. 184:3321. PubMed
5. Mattarollo S, et al. 2010. J Immunol. 184:1242. PubMed
6. Wood M, et al. 2011. Infect Immun. 79:708. PubMed
7. Munk R, et al. 2011. PLoS One. 6:e18553. PubMed
8. Fiorenza S, et al. 2012. J Immunol. 189:5622. PubMed
9. Janune D, et al. 2011. FEBS Lett. 585:3033. PubMed
10. Asgari E, et al. 2013. Blood. 122:3473. PubMed
11. Li D, et al. 2013. Clin Immunol. 149:411. PubMed
12. Bunse C, et al. 2013. PLoS One. 8:77925. PubMed
Antigen Details
Antigen References 1. Current Protocols in Immunology (John Wiley & Sons New York) Unit 6.24 Detection of Intracellular
Cytokines by Flow Cytometry (Barbara Foster and Calman Prussin NIAID NIH Bethesda MD).
2. Sander B, et al. 1991. Immunol. Rev. 119:65.
3. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
4. Prussin C, et al. 1995. J. Immunol. Meth. 188:117.
5. Jung T, et al. 1993. J. Immunol. Meth. 159:197.
Related Protocols
Intracellular Cytokine Staining Protocol - Video
Version: 1 Revision Date: 11/30/2012
Intracellular Flow Cytometry Staining Protocol
For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other
violations that may occur with the use of our products.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website,
www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to
manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written
approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses.
Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or
commercial use.
BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587
Catalog# / Size 420701 / 1 mL
Description Monensin is a protein transport inhibitor commonly used to enhance intracellular cytokine
staining signals by blocking transport processes during cell activation. Especially useful for the
intracellular staining of cytokines, monensin leads to the accumulation of most cytokines at
the Golgi Complex/Endoplasmic Reticulum (see Jung, et al., 1993). Optimal conditions for
use are cell type and time-dependent. Typically, protein transport inhibitors are included
during in vitro cell activation cultures for 4-24 hours prior to harvest (see references below for
additional information). Monensin Solution is supplied as a 1,000X solution, which should be
diluted to 1X in cell culture medium.
Product Details
Formulation Monensin Solution is supplied as a 1000X working solution in 70% Ethanol. Dilute to 1X in cell
culture medium.
Concentration 2.0 mM
Storage & Handling Store the solution between 2°C and 8°C. Monensin is known to be toxic; avoid direct body contact.
Ethanol is flammable; keep away from sources of fire.
Application ICFC - Quality tested
Recommended Usage Dilute the 1000X solution to 1X in the tissue culture medium. It is recommended that cells are
cultured with monensin for ≤ 24 hours, as this can become toxic for cell viability.
Application References
(PubMed link indicates
BioLegend citation)
1. Smeltz RB. 2007. J. Immunol. 178:4786.
2. Fuse S, et al. 2007. J. Immunol. 178:5227.
3. Durkin ET,et al. 2008. Blood 10:1182.PubMed
4. Durkin ET, et al. 2008. Blood 112:5245. PubMed
5. Liu XS, et al. 2009. J. Immunol. 183:51. PubMed
6. Mattarollo SR, et al. 2010. J. Immunol. 184:1242. PubMed
7. Yang M, et al. 2010. J. Immuonl. 184:3321. PubMed
8. Mattarollo SR, et al. 2010. J. Immunol. 184:5663. PubMed
9. Wood MW, et al. 2011. Infect Immun. 79:708. PubMed
10. Janune D, et al. 2011. FEBS Lett. 585:3033. PubMed
11. Fiorenza S, et al. 2012. J. Immunol. 189:5622. PubMed
12. Asgari E, et al. 2013. Blood. 122:3473. PubMed
Product Citations
1. Durkin E, et al. 2008. Blood. 112:5245. PubMed
2. Liu X, et al. 2009. J Immunol. 183:51:00. PubMed
3. Mattarollo S, et al. 2010. J Immunol. 184:5663. PubMed
4. Yang M, et al. 2010. J Immunol. 184:3321. PubMed
5. Mattarollo S, et al. 2010. J Immunol. 184:1242. PubMed
6. Wood M, et al. 2011. Infect Immun. 79:708. PubMed
7. Munk R, et al. 2011. PLoS One. 6:e18553. PubMed
8. Fiorenza S, et al. 2012. J Immunol. 189:5622. PubMed
9. Janune D, et al. 2011. FEBS Lett. 585:3033. PubMed
10. Asgari E, et al. 2013. Blood. 122:3473. PubMed
11. Li D, et al. 2013. Clin Immunol. 149:411. PubMed
12. Bunse C, et al. 2013. PLoS One. 8:77925. PubMed
Antigen Details
Antigen References 1. Current Protocols in Immunology (John Wiley & Sons New York) Unit 6.24 Detection of Intracellular
Cytokines by Flow Cytometry (Barbara Foster and Calman Prussin NIAID NIH Bethesda MD).
2. Sander B, et al. 1991. Immunol. Rev. 119:65.
3. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
4. Prussin C, et al. 1995. J. Immunol. Meth. 188:117.
5. Jung T, et al. 1993. J. Immunol. Meth. 159:197.
Related Protocols
Intracellular Cytokine Staining Protocol - Video
Version: 1 Revision Date: 11/30/2012
Intracellular Flow Cytometry Staining Protocol
For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other
violations that may occur with the use of our products.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website,
www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to
manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written
approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses.
Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or
commercial use.
BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587
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文献和实验相关实验
is lost on tips). Rotate in cold room for 1 hr. Spin and wash beads twice with RIPA (discard supernatant), and then once with Histone Wash Buffer. Add 30 µL of Histone Assay solution. Incubate at 37°C for exactly 30 min. Mix
Intracellular Cytokine Staining Protocol
.In contrast to detection of secreted cytokines by ELISA, for detection of intracellular cytokines, it is necessary to block secretion of cytokines with reagents such as Monensin or Brefeldin A during the last few hours of the stimulation. It is advised
Intracellular Cytokine Staining Protocol
hours stimulation. In contrast to detection of secreted cytokines by ELISA, for detection of intracellular cytokines, it is necessary to block secretion of cytokines with reagents such as Monensin or Brefeldin A during the last few hours
Monensin Solution (1,000X)
¥630
