- ￥209
- 碧云天（beyotime）
- P0013D
- 2025年11月27日
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- 规格：
100ml
碧云天生产的RIPA裂解液(RIPA Lysis Buffer)是一种传统的细胞组织快速裂解液。RIPA裂解液裂解得到的蛋白样品可以用于常规的PAGE、Western、免疫沉淀(immunol precipitation，IP)、免疫共沉淀(co-IP)和ELISA等。
本产品可以用于动物、植物的细胞或组织样品，也可以用于真菌或细菌样品。
RIPA的本意是Radio Immunoprecipitation Assay。RIPA裂解液的配方有很多种，根据其裂解液的强度大致可以分为强、中、弱三类。
RIPA裂解液(弱)的主要成分为50mM Tris(pH7.4)，150mM NaCl，1% NP-40，0.25% sodium deoxycholate，以及sodium orthovanadate，sodium fluoride，EDTA，leupeptin等多种抑制剂。可以有效抑制蛋白降解。
用RIPA裂解液裂解得到的蛋白样品，可以用碧云天生产的BCA蛋白浓度测定试剂盒(P0009/P0010/P0010S/P0011/P0012/P0012S)测定蛋白浓度。由于含有较高浓度的去垢剂，不能用Bradford法测定由本裂解液裂解得到样品的蛋白浓度。
包装清单：
| 产品编号 | 产品名称 | 包装 |
| P0013D | RIPA裂解液(弱) | 100ml |
| — | 说明书 | 1份 |
保存条件：
-20℃保存，一年有效。
注意事项：
为取得最佳的使用效果，尽量避免过多的反复冻融。可以适当分装后使用。
需自备PMSF。PMSF(ST506)可以向碧云天订购。也可以选购总体效果更佳的碧云天生产的P1045/P1046 蛋白酶磷酸酶抑制剂混合物(通用型, 50X)，或者根据具体用途选择P1048/P1049 蛋白酶磷酸酶抑制剂混合物(通用型, 质谱兼容, 50X)、P1050/P1051蛋白酶磷酸酶抑制剂混合物(哺乳动物样品抽提用, 50X)、P1055/P1056 蛋白酶磷酸酶抑制剂混合物(植物样品抽提用, 50X)、P1060/P1061 蛋白酶磷酸酶抑制剂混合物(真菌或酵母抽提用, 50X)、P1065/P1066 蛋白酶磷酸酶抑制剂混合物(细菌抽提用, 50X)。如果无需检测磷酸化蛋白，也可以选不含磷酸酶抑制剂的蛋白酶抑制剂化合物。
裂解样品的所有步骤都需在冰上或4℃进行。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
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文献和实验该产品被引用文献
1. Shen Y, Yang X, Dong N, Xie X, Bai X, Shi Y.
.
Generation and selection of immunized Fab phage display library against human B cell lymphoma.
Cell Res. 2007 Jul;17(7):650-60. (IF 20.507)
2. Yang R, Liu M, Liang H, Guo S, Guo X, Yuan M, Lian H, Yan X, Zhang S, Chen X, Fang F, Guo H, Zhang C.
.
miR-138-5p contributes to cell proliferation and invasion by targeting Survivin in bladder cancer cells.
Mol Cancer. 2016 Dec 15;15(1):82. (IF 15.302)
3. Xu WP, Yi M, Li QQ, Zhou WP, Cong WM, Yang Y, Ning BF, Yin C, Huang ZW, Wang J, Qian H, Jiang CF, Chen YX, Xia CY, Wang HY, Zhang X, Xie WF.
.
Perturbation of miR-370-LIN28A-NF-κB regulatory circuit contributes to the development of hepatocellularcarcinoma.
Hepatology. 2013 Dec;58(6):1977-91. (IF 14.679)
4. Yin C, Wang PQ, Xu WP, Yang Y, Zhang Q, Ning BF, Zhang PP, Zhou WP, Xie WF, Chen WS, Zhang X.
.
Hepatocyte nuclear factor-4α reverses malignancy of hepatocellular carcinoma through regulating miR-134 in theDLK1-DIO3 region.
Hepatology. 2013 Dec;58(6):1964-76. (IF 14.679)
5. Ting-Ting Liu, Xu-Fei Du, Bai-Bing Zhang, Hua-Xing Zi, Yong Yan, Jiang-An Yin, Han Hou, Shan-Ye Gu, Qi Chen, Jiu-Lin Du
.
Piezo1-Mediated Ca 2+ Activities Regulate Brain Vascular Pathfinding during Development
Neuron. 2020 Oct 14;108(1):180-192.e5. doi: 10.1016/j.neuron.2020.07.025. (IF 14.415)
6. Wang WT, Ye H, Wei PP, Han BW, He B, Chen ZH, Chen YQ.
.
LncRNAs H19 and HULC, activated by oxidative stress, promote cell migration and invasion incholangiocarcinoma through a ceRNA manner.
J Hematol Oncol. 2016 Nov 3;9(1):117. (IF 11.059)
7. Xujia Wu, Songhua Xiao, Maolei Zhang, Lixuan Yang, Jian Zhong, Bo Li, Fanying Li, Xin Xia, Xixi Li, Huangkai Zhou, Dawei Liu, Nunu Huang, Xuesong Yang, Feizhe Xiao, Nu Zhang
.
A novel protein encoded by circular SMO RNA is essential for Hedgehog signaling activation and glioblastoma tumorigenicity
Genome Biol. 2021 Jan 14;22(1):33. doi: 10.1186/s13059-020-02250-6. (IF 10.806)
8. Wang S, Li Y, Fan J, Wang Z, Zeng X, Sun Y, Song P, Ju D.
.
The role of autophagy in the neurotoxicity of cationic PAMAM dendrimers.
Biomaterials. 2014 Aug;35(26):7588-97. (IF 10.317)
9. Xie Q, Wang Z, Huang Y, Bi X, Zhou H, Lin M, Yu Z, Wang Y, Ni N, Sun J, Wu S, You Z, Guo C, Sun H, Wang Y, Gu P, Fan X.
.
Characterization of human ethmoid sinus mucosa derived mesenchymal stem cells (hESMSCs) and the application of hESMSCs cell sheets in bone regeneration.
Biomaterials. 2015 Oct;66:67-82. (IF 10.317)
10. Xie Q, Wang Z, Zhou H, Yu Z, Huang Y, Sun H, Bi X, Wang Y, Shi W, Gu P, Fan X.
.
The role of miR-135-modified adipose-derived mesenchymal stem cells in bone regeneration.
Biomaterials. 2016 Jan;75:279-94. (IF 10.317)
11. Lu Y, Xu S, Chen H, He M, Deng Y, Cao Z, Pi H, Chen C, Li M, Ma Q, Gao P, Ji Y, Zhang L, Yu Z, Zhou Z.
.
CdSe/ZnS quantum dots induce hepatocyte pyroptosis and liver inflammation via NLRP3 inflammasomeactivation.
Biomaterials. 2016 Jun;90:27-39. (IF 10.317)
12. Li H, Fan J, Sun L, Liu X, Cheng P, Fan H.
.
Functional regeneration of ligament-bone interface using a triphasic silk-based graft.
Biomaterials. 2016 Nov;106:180-92. (IF 10.317)
13. Zhou H, Yang C, Chen H, Li X, Li Y, Fan X.
.
A simple G-quadruplex molecular beacon-based biosensor for highly selective detection of microRNA.
Biosens Bioelectron. 2017 Jan 15;87:552-557. (IF 10.257)
14. Cong ZX, Wang HD, Zhou Y, Wang JW, Pan H, Zhang DD, Zhang L, Zhu L.
.
Temozolomide and irradiation combined treatment-induced Nrf2 activation increases chemoradiation sensitivity in human glioblastoma cells.
NEURO-ONCOLOGY. 2014 Jan;116(1):41-8. (IF 10.247)
15. Liu H, Ai J, Shen A, Chen Y, Wang X, Peng X, Chen H, Shen Y, Huang M, Ding J, Geng M.
.
c-Myc Alteration Determines the Therapeutic Response to FGFR Inhibitors.
Clin Cancer Res. 2017 Feb 15;23(4):974-984. (IF 10.107)
16. Li R, Yu C, Gao R, Liu X, Lu J, Zhao L, Chen X, Ding Y, Wang Y, He J.
.
Effects of DEHP on endometrial receptivity and embryo implantation in pregnant mice.
J Hazard Mater. 2012 Nov 30;241-242:231-40. (IF 9.038)
17. Zhou H, Lian C, Wang T, Yang X, Xu C, Su D, Zheng S, Huang X, Liao Z, Zhou T, Qiu X, Chen Y, Gao B, Li Y, Wang X, You G, Fu Q, Gurnett C, Huang D, Su P
.
MET mutation causes muscular dysplasia and arthrogryposis.
EMBO Mol Med. 2019 Mar 11(3). pii: e9709. (IF 8.821)
18. Yu X, Tang J, Wang Q, Ye W, Tao K, Duan S, Lu C, Yang X, Dong S, Zheng X, Wang Y.
.
The RxLR effector Avh241 from Phytophthora sojae requires plasma membrane localization toinduce plant cell death.
New Phytol. 2012 Oct;196(1):247-60. (IF 8.512)
19. Xu P, Zhang XX, Miao C, Fu Z, Li Z, Zhang G, Zheng M, Liu Y, Yang L, Wang T.
.
Promotion of melanoma cell invasion and tumor metastasis by microcystin-LR via phosphatidylinositol 3-kinase/AKT pathway.
Environ Sci Technol. 2013 Aug 6;47(15):8801-8. (IF 7.864)
20. Deng Z, Yan F, Jin Q, Li F, Wu J, Liu X, Zheng H.
.
Reversal of multidrug resistance phenotype in human breast cancer cells using doxorubicin-liposome-microbubble complexes assisted by ultrasound.
J Control Release. 2014 Jan 28;174:109-16. (IF 7.727)
21. Ling X, Zhang G, Chen Q, Yang H, Sun L, Zhou N, Wang Z, Zou P, Wang X, Cui Z, Liu J, Ao L, Cao J.
.
Shorter sperm telomere length in association with exposure to polycyclic aromatic hydrocarbons: Resultsfrom the MARHCS cohort study in Chongqing, China and in vivo animal experiments.
Environ Int. 2016 Oct;95:79-85. (IF 7.577)
22. Zhang CH, Hu SY, Cao MQ, Lin Y, Li YH.
.
Effects of Paeonol on the Expression of PTEN and AKT in Human MHCC97-H cells.
《现代生物医学进展》. 2007;7(8):1134-7.
23. Gong C, Liu Y, Xiao W, Yin J, Wang DH, Sheng H.
.
The Role of ERK1 /2 in Leptin Promoting the Proliferation of Human Endometrial Cancer Cell Line Ishikawa.
Chinese Journal of Cancer. 2007;26(11):1211-4. (IF 3.62)
24. Zhang X, Chen S, Wang Y.
.
Honokiol up-regulates prostacyclin synthease protein expression and inhibits endothelial cell apoptosis.
Eur J Pharmacol. 2007 Jan 5;554(1):1-7. (IF 3.263)
25. Zhang XT, Song TB, Du BL, Li DM, Li XM.
.
Caspase-3 antisense oligodeoxynucleotides inhibit apoptosis in gamma-irradiated human leukemia HL-60 cells.
Apoptosis. 2007 Apr;12(4):743-51. (IF 4.543)
26. Deng XQ, Chen LL, Li NX.
.
The expression of SIRT1 in nonalcoholic fatty liver disease induced by high-fat diet in rats.
Liver Int. 2007 Jun;27(5):708-15. (IF 5.175)
27. Dong S, Li C, Wu P, Tsien JZ, Hu Y.
.
Environment enrichment rescues the neurodegenerative phenotypes in presenilins-deficient mice.
Eur J Neurosci. 2007 Jul;26(1):101-12. (IF 3.115)
28. Zhang JQ, Zhao XK, He J, Zhu L, Wang XJ.
.
Expr ess and role of FGFR2 in bladder tr ansitional cell car cinoma.
《中国医学工程》. 2007 Sep;15(9):720-5.
29. Shi Y, Cheng SG, Chen X, Xiao CG.
.
Construction and identification of recombinant lentivirus-mediated gene transfer system for rat transducer of regulated CREB activity 1.
南京医科大学学报. 2008;22(5): 304-7.
30. Guo M, Huang T, Cui Y, Pan B, Shen A, Sun Y, Yi Y, Wang Y, Xiao G, Sun G.
.
PrPC interacts with tetraspanin-7 through bovine PrP154-182 containing alpha-helix 1.
BIOCHEM BIOPH RES CO. 2008 Jan 4;365(1):154-7. (IF 2.985)
31. Wang Y, Peng KM, Li JL, Song H, Li SH, Wei L, Wang JX.
.
Ultrastructure and melatonin 1a receptor distribution in the ovaries of African ostrich chicks.
Cytotechnology. 2008 Mar;56(3):187-95. (IF 1.777)
32. Luo Y, Waladali W, Li S, Zheng X, Hu L, Zheng H, Hu W, Chen C.
.
17beta-Estradiol affects proliferation and apoptosis of rat prostatic smooth muscle cells by modulating cell cycle transition and related proteins.
Cell Biol Int. 2008 Aug;32(8):899-905. (IF 2.571)
33. Wei Y, Weng D, Li F, Zou X, Young DO, Ji J, Shen P.
.
Involvement of JNK regulation in oxidative stress-mediated murine liver injury by microcystin-LR.
Apoptosis. 2008 Aug;13(8):1031-42. (IF 4.543)
34. Tang Z, Yang J, Liu X, Wang Y, Peng S.
.
Polyaspartoyl.L-arginine enhances prostacyclin synthesis in rat aortic endothelial cells.
Eur J Pharmacol. 2008 Dec 28;601(1-3):124-8. (IF 3.263)
35. Li SN, Wang X, Zeng QT, Feng YB, Cheng X, Mao XB, Wang TH, Deng HP.
.
Metformin inhibits nuclear factor kappaB activation and decreases serum high-sensitivity C-reactive protein level in experimental atherogenesis of rabbits.
Heart Vessels. 2009;24(6):446-53. (IF 1.618)
36. Wang S, Sun X, Ding F, Zhang K, Zhao R, Li S, Li R, Tang B, Zhang L, Liu Y, Li J, Gao F, Wang H, Wang L, Dai Y, Li N.
.
Removal of selectable marker gene from fibroblast cells in transgenic cloned cattle by transient expression of Cre recombinase and subsequent effects on recloned embryo development.
Theriogenology. 2009;72(4):535-41. (IF 2.094)
37. Zhang SP, Wu YW, Wu ZZ, Liu HY, Nie JH, Tong J.
.
Up-regulation of RAGE and S100A6 in rats exposed to cigarette smoke.
Environmental Toxicology and Pharmacology. 2009;28:259–264. (IF 3.061)
38. Chen Y, Wang J, Yao Y, Yuan W, Kong M, Lin Y, Geng D, Nie R.
.
CRP regulates the expression and activity of tissue factor as well as tissue factor pathwayinhibitor via NF-kappaB and ERK 1/2 MAPK pathway.
FEBS Lett. 2009;583(17):2811-8. (IF 3.057)
39. Li J, Tian T, Wang XY, Li F, Ren GS.
.
Expression and Clinical Signifi cance of REGγ in Gastric Cancer Tissue and Variously Differentiated Gastric Cancer Cell Lines.
Clinical Oncology and Cancer Research. 2009;6:208-213.
40. Yu X, Huang Y, Hu Q, Ma L.
.
Hyperhomocysteinemia stimulates hepatic glucose output and PEPCK expression.
ACTA BIOCH BIOPH SIN. 2009;41(12):1027-32. (IF 2.836)
41. Xiao X, Liu A, Wen H, Tian Y, Ni J, Liu G.
.
Expression and localization of transcription factor Ets-1 in the rat ovary during the estrous cycle and pregnancy.
Fertil Steril. 2009;91(5 Suppl):1998-2005. (IF 6.312)
42. Nie W, Yan H, Li S, Zhang Y, Yu F, Zhu W, Fan F, Zhu J.
.
Angiotensin-(1-7) enhances angiotensin II induced phosphorylation of ERK1/2 in mouse bone marrow-derived dendritic cells.
Mol Immunol. 2009 Jan;46(3):355-61. (IF 3.641)
43. Lv J, Jia R, Yang D, Zhu J, Ding G.
.
Candesartan attenuates Angiotensin II-induced mesangial cell apoptosis via TLR4/MyD88 pathway.
BIOCHEM BIOPH RES CO. 2009 Feb 27;380(1):81-6. (IF 2.985)
44. Gao R, Wang J, Shen Y, Lei M, Wang Z.
.
Functional expression of voltage-gated sodium channels Nav1.5 in human breast caner cell line MDA-MB-231.
J HUAZHONG U SCI-MED. 2009 Feb;29(1):64-7. (IF 1.151)
45. Fang Z, Wang Q, Cao W, Feng Q, Li C, Xie L, Zhang R.
.
Investigation of phosphorylation site responsible for CaLP (P. fucata) nucleo-cytoplasmic shuttling triggered by overexpression of p21Cip1.
MAR BIOTECHNOL. 2009 Mar-Apr;11(2):270-9. (IF 2.798)
46. Waladali W, Luo Y, Li WS, Zheng MX, Hu QL.
.
17Beta-estradiol affects the proliferation and apoptosis of rat bladder neck smooth muscle cells by modulating cell cycle transition and related proteins.
World J Urol. 2009 Apr;27(2):241-8. (IF 3.217)
47. Qin ZX, Zhu HY, Hu YH.
.
Effects of lysophosphatidylcholine on beta-amyloid-induced neuronal apoptosis.
Acta Pharmacol Sin. 2009 Apr;30(4):388-95. (IF 5.064)
相关实验
1ml RIPA buffer contains 35μl proteinase inhibitor(sigma cat no P8340) and 10μl phosphatase inhibitorRIPA buffer 最终浓度1M Tris-HCl pH7.5) 5 ml 50mMNaCl 0.87g 0.15 MNa-deoxycholale 0.1g （1%）0.5M EDTA(pH8.0) 0.8mlNaF 41 mgNonidet P40 1 ml （1%）Aprotinin
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RIPA裂解液(弱)
￥209
