Propidium Iodide Solution

Propidium Iodide Solution
Catalog# / Size 421301 / 2 mL
Other Names PI
Description Propidium iodide (PI) is a fluorescent dye that binds to DNA. When excited by 488nm laser
light, it can be detected with in the PE/Texas Red® channel with a bandpass filter 610/10. It
is commonly used in evaluation of cell viability or DNA content in cell cycle analysis by flow
cytometry.
Product Details
Formulation Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide.
Concentration 0.5 mg/ml
Storage & Handling The solution should be stored undiluted between 2°C and 8°C, and protect from light. Caution: This
solution contains hazardous material; handle with care.
Application FC - Quality tested
Recommended Usage The suggested use of this solution for viability staining is 10 µl per million cells in 0.5 ml/test, and
incubate for 15 minutes at 4 °C before analysis. For Cell Cycle analysis, please see our Propidium
Iodide Cell Cycle Staining Protocol. Caution: This solution is toxigenic and mutagenic; handle with
care.
Excitation Laser Blue Laser (488 nm)
Application Notes Propidium Iodide Solution can be used in evaluation of apoptosis, cell viability and cell cycle analysis
by flow cytometry.
Application References
(PubMed link indicates
BioLegend citation)
1. Vermes I, et al.1995. J. Immunol. Methods 184:39.
2. Darzynkiewicz Z, et al. 1992. Cytometry 13(8):795.
3. Douglas RS, et al. 1995. J. Immunol. Methods 188:219.
4. Sakimoto I, et al. 2006. Cancer Research 66:2287.PubMed
5. Liu H, et al. 2012. Int J Biochem Cell Biol. 45:408. PubMed
6. Juel HB, et al. 2013. PLoS One. 8:64619. PubMed
7. Ren Q, et al. 2013. PLoS One. 8:74732. PubMed
8. Zhao W, et al. 2013. Clin Immunol. 149:119. PubMed
9. Beggs KM, et al. 2014. Toxicol Sci. 137:91. PubMed
10. Ewald SE, et al. 2014. Infect Immun. 82:460. PubMed
11. Tsou WI, et al. 2014. J Biol Chem. 289:25750. PubMed
12. Kong S, et al. 2014. J Immunol. 193:5515. PubMed
Product Citations
1. Sakimoto I, et al. 2006. Cancer Res. 66:2287. PubMed
2. Liu H, et al. 2012. Int J Biochem Cell Biol. 45:408. PubMed
3. Juel H, et al. 2013. PLoS One. 8:64619. PubMed
4. Ren Q, et al. 2013. PLoS One. 8:74732. PubMed
5. Zhao W, et al. 2013. Clin Immunol. 149:119. PubMed
6. Beggs K, et al. 2014. Toxicol Sci. 137:91. PubMed
7. Ewald S, et al. 2014. Infect Immun . 82:460. PubMed
8. Tsou W, et al. 2014. J Biol Chem. 289:25750. PubMed
9. Kong S, et al. 2014. J Immunol. 193:5515. PubMed
10. Sapanidou V, et al. 2015. Theriogenology. . PubMed
11. Mensen A, et al. 2015. Biol Blood Marrow Transplant. Available online 10 August 2015. PubMed
12. Bao W, et al. 2016. Sci Rep. 6:22579. PubMed
Antigen Details
Biology Area Cell Biology, Cell Cycle/DNA Replication, Neuroscience
Related Protocols
Propidium Iodide Cell Cycle Staining Protocol
Version: 2 Revision Date: 10/08/2015 
Cell Surface Flow Cytometry Staining Protocol
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