- ¥700
- Biolegend
- 420801
- 进口
- 2025年07月15日
12 年金牌会员
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存：
现货
- 英文名：
Fixation Buffer
- 供应商：
上海善然生物科技有限公司
- 保存条件：
低温
- 规格：
100 mL
Fixation Buffer
Catalog# / Size 420801 / 100 mL
Other Names Fixative, Paraformaldehyde
Description Fixation Buffer is useful for intracellular staining procedures, e.g., in preparation of cells for
staining intracellular cytokines or other proteins. Fixation Buffer is used to fix cells prior to
permeabilization using Permeabilization Wash Buffer (Cat. No. 421002). BioLegend's
Fixation Buffer has been formulated with prescreened paraformaldehyde with low
background, thus producing the greatest signal to noise ratio.
Product Details
Storage & Handling This buffer solution should be stored between 2°C and 8°C.
Application ICFC - Quality tested
ICC
Recommended Usage For cell fixation, use 0.5 ml fixation buffer per tube and leave it in the dark for 20 minutes at room
temperature. It is recommended that the reagent be titrated for optimal performance for each
application. For the fixation procedure, please refer to the "Intracellular Cytokine Staining Protocol"
under "Support" on BioLegend's website.
Caution: This buffer contains paraformaldehyde, which is toxigenic and mutagenic. Please handle
with caution and wear gloves, lab coat and necessary protection to avoid direct body contacts.
Application Notes This 1X PBS solution contains 4% paraformaldehyde, which is toxic and is a suspected carcinogen.
Contact with eyes, skin and mucous membranes should be avoided.
Additional Product Notes View more applications data using this product to stain Veri-Cells™ lyophilized control cells and to
perform phospho-flow in our Scientific Poster Library.
Application References
(PubMed link indicates
BioLegend citation)
1. Kang YJ, et al. 2007. Nature Immunol. 8:601.
2. Kenna TJ, et al. 2010. J. Immunol. 184:598. PubMed
3. Sullivan BP, et al. 2010. Am J Pathol. 177:2837. PubMed
4. del Rio ML, et al. 2011.Transplantation. 92:1085. PubMed
5. del Rio ML, et al. 2012. J. Immunol. 188:4885. PubMed
6. Marongiu L, et al. 2013. PLoS One. 8:75684. PubMed
7. Haberthur K, et al. 2013. J Virol. 87:11751. PubMed
8. Busskamp V, et al. 2014. Mol Syst Biol. 10:760. PubMed
Product Citations
1. Sullivan B, et al. 2010. Am J Pathol. 177:2837. PubMed
2. Kenna T, et al. 2010. J Immunol. 184:598. PubMed
3. Rio M, et al. 2012. J Immunol. 188:4885. PubMed
4. Rio M, et al. 2011. Transplantation. 92:1085. PubMed
5. Marongiu L, et al. 2013. PLoS One. 8:75684. PubMed
6. Haberthur K, et al. 2013. J Virol. 87:11751. PubMed
7. Busskamp V, et al. 2014. Mol Syst Biol. 10:760. PubMed
8. Wright A, et al. 2015. Immunobiology. 220: 859-864. PubMed
9. Clancy-Thompson E, et al. 2015. Cancer Immunol Res. 3: 956-967. PubMed
10. Ede J, et al. 2015. Toxicol Sci. 148: 108 - 120. PubMed
11. Glaser K, et al. 2016. PLoS One. 11: 0146898. PubMed
12. Guye P, et al. 2016. Nat Commun. 7:10243. PubMed
Antigen Details
Antigen References 1. Current Protocols in Immunology (John Wiley & Sons New York) Unit 6.24 Detection of Intracellular
Cytokines by Flow Cytometry (Barbara Foster and Calman Prussin NIAID NIH Bethesda MD).
2. Sander B, et al. 1991. Immunol. Rev. 119:65.
3. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
4. Prussin C, et al. 1995. J. Immunol. Meth. 188:117.
Related Protocols
Intracellular Cytokine Staining Protocol - Video
Version: 3 Revision Date: 01/26/2015
Intracellular Flow Cytometry Staining Protocol
For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other
violations that may occur with the use of our products.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website,
www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to
manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written
approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses.
Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or
commercial use.
BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587
Catalog# / Size 420801 / 100 mL
Other Names Fixative, Paraformaldehyde
Description Fixation Buffer is useful for intracellular staining procedures, e.g., in preparation of cells for
staining intracellular cytokines or other proteins. Fixation Buffer is used to fix cells prior to
permeabilization using Permeabilization Wash Buffer (Cat. No. 421002). BioLegend's
Fixation Buffer has been formulated with prescreened paraformaldehyde with low
background, thus producing the greatest signal to noise ratio.
Product Details
Storage & Handling This buffer solution should be stored between 2°C and 8°C.
Application ICFC - Quality tested
ICC
Recommended Usage For cell fixation, use 0.5 ml fixation buffer per tube and leave it in the dark for 20 minutes at room
temperature. It is recommended that the reagent be titrated for optimal performance for each
application. For the fixation procedure, please refer to the "Intracellular Cytokine Staining Protocol"
under "Support" on BioLegend's website.
Caution: This buffer contains paraformaldehyde, which is toxigenic and mutagenic. Please handle
with caution and wear gloves, lab coat and necessary protection to avoid direct body contacts.
Application Notes This 1X PBS solution contains 4% paraformaldehyde, which is toxic and is a suspected carcinogen.
Contact with eyes, skin and mucous membranes should be avoided.
Additional Product Notes View more applications data using this product to stain Veri-Cells™ lyophilized control cells and to
perform phospho-flow in our Scientific Poster Library.
Application References
(PubMed link indicates
BioLegend citation)
1. Kang YJ, et al. 2007. Nature Immunol. 8:601.
2. Kenna TJ, et al. 2010. J. Immunol. 184:598. PubMed
3. Sullivan BP, et al. 2010. Am J Pathol. 177:2837. PubMed
4. del Rio ML, et al. 2011.Transplantation. 92:1085. PubMed
5. del Rio ML, et al. 2012. J. Immunol. 188:4885. PubMed
6. Marongiu L, et al. 2013. PLoS One. 8:75684. PubMed
7. Haberthur K, et al. 2013. J Virol. 87:11751. PubMed
8. Busskamp V, et al. 2014. Mol Syst Biol. 10:760. PubMed
Product Citations
1. Sullivan B, et al. 2010. Am J Pathol. 177:2837. PubMed
2. Kenna T, et al. 2010. J Immunol. 184:598. PubMed
3. Rio M, et al. 2012. J Immunol. 188:4885. PubMed
4. Rio M, et al. 2011. Transplantation. 92:1085. PubMed
5. Marongiu L, et al. 2013. PLoS One. 8:75684. PubMed
6. Haberthur K, et al. 2013. J Virol. 87:11751. PubMed
7. Busskamp V, et al. 2014. Mol Syst Biol. 10:760. PubMed
8. Wright A, et al. 2015. Immunobiology. 220: 859-864. PubMed
9. Clancy-Thompson E, et al. 2015. Cancer Immunol Res. 3: 956-967. PubMed
10. Ede J, et al. 2015. Toxicol Sci. 148: 108 - 120. PubMed
11. Glaser K, et al. 2016. PLoS One. 11: 0146898. PubMed
12. Guye P, et al. 2016. Nat Commun. 7:10243. PubMed
Antigen Details
Antigen References 1. Current Protocols in Immunology (John Wiley & Sons New York) Unit 6.24 Detection of Intracellular
Cytokines by Flow Cytometry (Barbara Foster and Calman Prussin NIAID NIH Bethesda MD).
2. Sander B, et al. 1991. Immunol. Rev. 119:65.
3. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
4. Prussin C, et al. 1995. J. Immunol. Meth. 188:117.
Related Protocols
Intracellular Cytokine Staining Protocol - Video
Version: 3 Revision Date: 01/26/2015
Intracellular Flow Cytometry Staining Protocol
For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other
violations that may occur with the use of our products.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website,
www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to
manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written
approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses.
Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or
commercial use.
BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
文献和实验相关实验
【求助】shRNA连接时，如果没有annealing buffer,可不可以用PCR buffer?
bobbymm shRNA要做连接的时候发现 annealing buffer 没有了。可以用 PCR buffer代替不？ zhujoker 就用普通的去离子水就可以了，没有那么复杂。不要想当然得使用PCR buffer。 bobbymm 用的是TAKARA的PCR BUFFER ，连接的还不错。 已经送去测序了。 谢谢回复。 本文
当某溶液加入酸或碱时，具有使 pH变化减弱的作用，换言之，此时的 pH变化比纯水小时，称此作用为缓冲作用，称具有这种作用的系统为缓冲系统。现假定被测溶液 1000毫升中加入 dB克当量的强酸或强碱，其 pH仅作 dpH变化，那么缓冲作用的大小，其比为： β为缓冲价或缓冲系数，而 dpH值在当溶液呈碱性变化时为正，呈酸性变化时为负，所以为了使β为正数，而 dB在加碱时取正值，加酸时为负值。
Methanol Fixation for Immunofluorescence
. We recommend that you try both types of fixation for your particular antibody/fusion protein when working out your initial immunofluorescence conditions. MeS Buffer 100 mM MeS, pH 6.9 1 mM EGTA 1 mM MgCl2 Store at 4°C Methanol Fix (100
技术资料暂无技术资料 索取技术资料
Fixation Buffer
¥700
