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- 2025年10月09日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存：
10
- 英文名：
Opti-MEM™ I Reduced Serum Medium
- 保质期：
1年
- 供应商：
朗智（上海）生物科技有限公司
- 保存条件：
2-8°C
- 规格：
100ml
Description
Opti-MEM® I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology. Opti-MEM® I medium is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM® I medium can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. We offer a variety of Opti-MEM® I modifications for a range of cell culture applications.This Opti-MEM® I is modified as follows:
| With |
| • L-glutamine |
| • Phenol Red |
The complete formulation is confidential. For more information, please contact Technical Services.
Using Opti-MEM® I Medium
Opti-MEM® I Reduced-Serum Medium is a unique medium that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%. Opti-MEM® I medium uses a sodium bicarbonate buffer system (2.4 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
cGMP manufacturing and quality system
Opti-MEM® I is manufactured at a cGMP-compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Opti-MEM® I product made in our Grand Island facility (31985-070). This facility is also registered with the FDA as a medical device manufacturer and certified to the ISO 13485 standards.
Specifications
| Form: | Liquid |
|---|---|
| Glutamine: | L-Glutamine |
| Green Features: | Sustainable packaging |
| Phenol Red Indicator: | Phenol Red |
| Product Line: | Gibco®,Opti-MEM® |
| Product Size: | 10 x 500 mL |
| Shipping Condition: | Room Temperature |
| Volume (Metric): | 500 ml |
Contents & storage
Storage conditions: 2-8° C. Protect from lightShipping conditions: Ambient
Shelf life: 18 months from date of manufacture
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
更多详情请联系：
朗智（上海）生物科技有限公司
http://lzbiotech.com
Tel: +(86)021-54019192
Mobile: +(86) 13564617637（微信同号）
QQ：370323648
Email: songxinwei@lzbiotech.com
地址: 上海市徐汇区中山南二路777弄1号楼2405室
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文献和实验Culturing Human Embryonic Stem Cells (hESCs) on MEF-Conditioned Medium
实验试剂 Dulbecco’s Modified Eagle Medium (D-MEM) Fetal Bovine Serum (FBS), ESC-Qualified D-MEM/F-12 (1X) with GlutaMAX™-I KnockOut™ Serum Replacement (KSR) MEM Non-Essential Amino Acids Solution, 10 mM
Growing Cells in Geltrex Reduced Growth Factor Basement Membrane Matrix
bubbles. 3) Dilute the extract to desired concentration in cold serum-free medium. Empirical determination of the optimal coating concentration for your application may be required. A protein concentration of 0.1 mg/ml is a recommended
分析? 各位前辈：我想把microRNA转到成纤维细胞内，用的是GIBCO(tm) Opti-MEM I Reduced-Serum Medium (1X)和Lipofectamine™ 2000转的。现在的问题是一加转染试剂细胞就大量死亡。Opti-MEM和冲洗用的PBS都37度预热过的。甚至我用无血清的DMEM对照也大量死亡。有时细胞死亡稍微好点，是不是和细胞批次关系更密切，还是有其他原因。 我的实验操作 ： 1 10%血清DMEM接种细胞在24孔板，长至30~50%满底
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