Fluorescein-5-thiosemicarbazid

Fluorescence Assays for Measuring Fatty Acid Binding and Transport Through Membranes

; (2) the naturally occurring fluorescent cis -parinaric acid, which specifically measures the insertion of the FA acyl chain into the hydrophobic core of the phospholipid bilayer, and (3) a fluorescein-labeled phospholipid (N-fluorescein-5-thiocarbomoyl

Homogenous Detection of Nucleic Acids Using Self-Quenched Polymerase Chain Reaction Primers Labeled With a Single Fluorophore (LUX Primers)

product. The hairpin oligonucleotides (ΔG from -1.6 to -5.8 kcal/mol) are as efficient as linear primers and provide additional specificity to the PCR by preventing primer-dimers and mispriming. Self-quenched primers could be designed manually

荧光素

荧光是指一个分子或原子吸收了给予的能量后即刻引起发光，停止能量供给，发光也瞬时停止（一般持续10-7～10-8s）。可以产生明亮荧光的染料物质，称荧光色素。目前主要常用的荧光色素有以下3种： （一）异硫氰酸荧光素（Fluorescein isothiocyanate, GITC） 呈黄色、橙黄或褐黄色粉末或结晶，性质稳定，在室温下能保存2年以上，在低温中可保存多年。易溶于水和酒精。最大吸收光谱为490～495nm，最大