pEBMulti-Puro

常用载体元件介绍及病毒载体应用

子CaMKIIa，星型胶质细胞特异性启动子GfaABC1D，心肌特异性启动子cTNT，肝实质细胞特异性启动子TBG等。(2) 荧光：针对编码蛋白，常规推荐目的基因和荧光蛋白非融合表达，通过荧光观察感染效率。若需要用荧光标记目的基因的位置，如亚细胞定位等实验时可考虑做目的基因和荧光蛋白融合表达。因蛋白均有自己的空间结构，若选择融合表达，两个蛋白可能会相互影响。融合表达常用结构：非融合表达常用结构：(3) 抗性：A 常用原核抗性：Amp，Kan。B 常用真核抗性：Puro，blasticidin(BSD，BSR

Retroviral infection of primary human keratinocytes (PHKs)

to be low, infect again today and begin selection on day 5 Selection of PHKs G418 only : use 150µg/mL in KGM. Check each day. Puro only : try 1µg/mL G418 + Puro : try 50µg/mL G418 + 0.4µg/mL puro

Construction of Simple and Efficient DNA Vector-Based Short Hairpin RNA Expression Systems for Specific Gene Silencing in Mammalian Cells

. Moreover, to improve the constructing and screening efficiency for the shRNA-expression recombinant clones, these four DNA vectors are further reconstructed by inserting a stuffer of puromycin resistance gene (Puro R ) between restriction enzyme Cla I and Hind III