优势: 1.两型孢毛霉培养供货及时。我们订货周期短,运输时间短,在zui短时间内为您提供所需产品。 2.价格优惠:我公司是ScienCell和美国ATCC的中国代理商,没有中间环节。在国内我们保证价格zui低。 3.服务保障:我们拥有专用的技术指导团队,对每售出的一件产品,提供全面的售前、售中和售后服务。 4.产品种类齐全:25个系统共150多种细胞,满足不同客户的要求。细胞经过严格的质控,纯度可达98%。常用产品备有现货。其产品类型如下:消化系统细胞株,呼吸系统细胞株,泌尿系统细胞株生殖系统细胞株 血液循环系统 内皮细胞细胞株 神经系统及其他细胞株,动物细胞株和菌株 5.对于部分难培养的细胞我公司可代复苏细胞,并提供。 6.经验丰富:我公司是国内专业化的细胞销售公司,产品仅用于科研实验具有十余年细胞产品销售经验,有专业的销售团队和技术人员。 保藏方法与步骤: 一)培养基制备 1.准备器皿,如三角瓶、试管、培养皿、烧杯、吸管等,根据不同情况洗涤、干燥、包装、灭菌。 2.溶解培养基配料:先在烧杯中放适量水,按培养基配方称取各项材料,依次将缓冲化合物、主要元素、微量元素、维生素等材料加入水中溶解,最后加足水量。 3.调pH值; 4.加凝固剂,如琼脂、明胶等。将凝固剂加入液体培养基中,加热并不断搅拌至融解,再补足所蒸发水分。 5.过滤分装; 6.包扎标记; 7.灭菌; 8.无菌检查。 二)接种 1.斜面接种 根据菌种的不同性征,可选择不同的方法,如点接、中央划线、稀波状蜿蜒划线法、密波状蜿蜒划线法、挖块接种法等; 2.穿刺接种 用直接种针从原菌种斜面上挑取少量菌苔,从柱状培养基中心自上而下刺入,直到接近管底(勿穿到管底),然后沿原穿刺途径慢慢抽出接种针。适用于细菌和酵母菌等。 3.液体接种 挑取少量固体斜面菌种或用无菌滴管等吸取原菌液接种于新鲜液体培养基中。 H-Thr(Bzl)-Obzl Hydrochloride118584-54-6规格含量:0.5mgChemical Name:Vitamin D3-d6Synonyms:(3β,5Z,7E)-9,10-Secocholesta-5,7,10(19)-trien-3-ol-d6; Cholecalciferol-d6; Duphafral D3 1000-d6; Delsterol-d6; Deparal-d6; Ebivit-d6; Oleovitamin D3-d6; Provitina-d6; Ricketon-d6; Vi-De3-d6; Videkhol-d6; Vigorsan-d6; Vitamin D3-d6;CAS Number:118584-54-6Molecular Formula:C₂₇H₃₈D₆OMolecular Weight:390.67Category:Standards; Vitamin Derivatives; Vitamin D Derivatives;Applications:Isotope labelled vitamin d3, the vitamin that mediates intestinal calcium absorbtion, bone calcium metabolism and probably, muscle activity. Occurs in and is isolated from fish liver oils. Vitamin D acts through a receptor that is a member of the ligand-dependent transcription factor superfamily. Modulates the proliferation and differentiation of both normal and cancer cells. Has antiproliferative and antimetastatic effects on breast, colon, and prostate cancer cells. Activated vitamin D receptors in intestine and bone maintain calcium absorbance and homeostasis. 6,7,8,9-tetrahydro-5H-pyrido[2,3-b]azepine规格含量:10mgChemical Name:6Z,10Z-Vitamin K2Synonyms:2-Methyl-3-((2E,6Z,10Z)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl)naphthalene-1,4-dione; 6Z,10Z-Vitamin K2(20)Molecular Formula:C₃₁H₄₀O₂Molecular Weight:444.65Category:Standards; Pharmaceutical/API Drug Impurities/Metabolites;Applications:6Z,10Z-Vitamin K2 is the 6Z,10Z-isomer of Vitamin K2 (M218595); the most common type of Vitamin K2, known as a hemostatic agent and used as adjunctive therapy for the pain of osteoporosis. Thymidine-1',2',3',4',5'-13C587649-56-7规格含量:5gChemical Name:Vitamin D3 O-t-Butyldimethylsilyl EtherSynonyms:(1,1-Dimethylethyl)dimethyl[[(3β,5Z,7E)-9,10-secocholesta-5,7,10(19)-trien-3-yl]oxy]-silane; 9,10-Secocholestane Silane Derivative; t-Butyldimethyl(((S,Z)-3-((E)-2-((1R,3aS,7aR)-7a-methyl-1-((R)-6-methylheptan-2-yl)hexahydro-1H-inden-4(2H)-ylidene)ethylidene)-4-methylenecyclohexyl)oxy)silane;CAS Number:87649-56-7Molecular Formula:C₃₃H₅₈OSiAppearance:Clear OilMolecular Weight:498.9Solubility:DCM, Hexanes Thiazole104846-62-0规格含量:5gChemical Name:Vitamin D2 O-t-Butyldimethylsilyl EtherSynonyms:(1R,3aS,4E,7aR)-4-[(2Z)-2-[(5S)-5-[[(1,1-Dimethylethyl)dimethylsilyl]oxy]-2-methylenecyclohexylidene]ethylidene]octahydro-7a-methyl-1-[(1R,2E,4R)-1,4,5-trimethyl-2-hexen-1-yl]-1H-indene; (1,1-Dimethylethyl)[[(3β,5Z,7E,22E)-9,10-secoergosta-5,7,10(19),22-tetraen-3-yl]oxy]dimethyl-silane;CAS Number:104846-62-0Molecular Formula:C₃₄H₅₈OSiAppearance:Light Yellow OilMolecular Weight:510.91Solubility:DCM, Hexanes L-Thr-NHMe Hydrochloride28152-73-0规格含量:10mgChemical Name:Vincaminic AcidSynonyms:(3α,14β,16α)-14,15-Dihydro-14-hydroxyeburnamenine-14-carboxylic Acid; (+)-Vincaminic Acid; (+)-cis-Vincaminic Acid; cis-Vincaminic Acid; Eburnamenine-14-carboxylicCAS Number:28152-73-0Molecular Formula:C₂₀H₂₄N₂O₃Molecular Weight:340.42Category:Standards; Pharmaceutical/API Drug Impurities/Metabolites;Applications:Vincaminic Acid is a derivative compound of Vincamine (V314030), that enhances cerebral circulation and sinus flow in dogs . Vincamine is often used as a nootropic agent to combat the effects of aging, or in conjunction with other nootropics (such as piracetam) for a variety of purposes. Vincamine is a peripheral vasodilator that increases blood flow to the brain. 4-((Thiomorpholino)methyl)phenylboronic Acid Pinacol Ester规格含量:25mgChemical Name:Vamidothion-d6 SulfoxideSynonyms:Phosphorothioic Acid O,O-(Dimethyl-d6) S-[2-[[1-Methyl-2-(methylamino)-2-oxoethyl]Alternate CAS #:20300-00-9-unlabelledMolecular Formula:C₈H₁₂D₆NO₅PS₂Molecular Weight:309.37Category:Standards; Isotopic Labeled Analogues;Applications:Labelled Vamidothion sulfoxide (V756500). Vamidothion sulfoxide is a metabolite of Vamidothion, a phosphorothioic insecticide for apples and potatoes. 4-(2-O-Thp-hydroxy-ethyl)-phenyl-boronic Acid63405-32-3规格含量:50mgChemical Name:5-Vinylpyridine-2-carbonitrileCAS Number:63405-32-3Molecular Formula:C₈H₆N₂Molecular Weight:130.14Category:Building Blocks; Miscellaneous;Applications:5-VINYLPYRIDINE-2-CARBONITRILE (cas# 63405-32-3) is a useful research chemical. Thieno[2,3-C]pyridine-3-carboxylic Acid858134-23-3规格含量:10mgChemical Name:Vialinin ASynonyms:Terrestrin A; Vialinin ACAS Number:858134-23-3Molecular Formula:C₃₄H₂₆O₈Molecular Weight:562.57Category:Standards; Enzyme Activators and Inhibitors; Pharmaceutical/API Drug Impurities/Metabolites;Applications:Vialinin A is a Potent Inhibitor of TNF-α production. Vialinin A is a potent antioxidant. 两型孢毛霉培养Gibco 17101015 Collagenase Type II 1g 人心肌细胞总RNAHCM NA LAMP2 Others Cynomolgus 食蟹猴 LAMP2 人细胞裂解液 (阳性对照) AsPc-1细胞,人转移胰腺癌细胞 人正常皮肤细胞,HaCaT细胞 小鼠T淋巴细胞;Cl.Ly1+2-/9 HSC-T6(大鼠肝星形细胞) 5×106cells/瓶×2 豚鼠胚胎细胞;104C1 phospho-CSF1R(Tyr923) 磷酸化巨噬细胞集落刺激因子受体抗体规格: 0.1ml MCT1 单羧酸转运蛋白-1抗体规格: 0.2ml MCM2 微小染色体维持缺陷蛋白2抗体规格: 0.2ml MCM3 微小染色体维持缺陷蛋白3抗体规格: 0.2ml MCM5 微小染色体维持缺陷蛋白5抗体规格: 0.1ml