肝S9

产品规格：0.5mL、1mL、2mL、5mL；  用途：Ames实验、CA实验等遗传毒性分析      
      肝S9是肝组织匀浆经过9000g离心后的上清部分，简称为S9。S9是完整肝细胞去除细胞核、线粒体及大的碎片之后的部分，原则上保留了定位于内质网上的微粒体蛋白，溶解在细胞浆液中的蛋白，以及各种细胞内容物质，如NADPH、UDPGA、PAPS等启动药物代谢反应的辅助因子。S9可以扩展微粒体研究的范围，但是引起所含有的辅助因子（也称辅酶）量少，实验时还是需要添加这些因子，以便实验顺利开展。
      凭借先进的技术设备和资深的专业化技能经验，欣润生物研发中心除拥有人、小鼠、大鼠肝S9、肝微粒体和细胞浆产品外，我们还可以为您提供特定物种、特定模型和特定年龄等非常规样品的肝S9、肝微粒体和细胞浆产品的定制服务。

   

我们提供的肝S9、肝微粒体和细胞浆定制服务具有以下优势：
一、具有多年肝S9、肝微粒体和细胞浆分离提纯经验，技术路线成熟可靠；
二、定制服务周期短：正常情况下，一般仅需2-3周时间；
三、严格的质量检测：欣润生物提供的肝S9、肝微粒体和细胞浆均经过严格的质量检测和结果分析，并列出详细的实验步骤；
四、完善的售后服务：我们不仅提供给您优质的产品，同时还可以为您提供专业的技术支持，确保您正确使用定制化的肝S9、肝微粒体和细胞浆。
   

Stepwise Targeted Matching Strategy for Comprehensive Profiling of Xanthohumol Metabolites In Vivo and In Vitro Using UHPLC-Q-Exactive Orbitrap Mass Spectrometer

Xanthohumol (XN), a natural prenylated flavonoid extracted and isolated from the hop plant (Humulus lupulus), possesses diverse pharmacological activities. Although the metabolites of XN have been investigated in the previous study, a comprehensive metabolic profile has been insufficient in vivo or in vitro until now. The current study was aimed at systematically elucidating the metabolic pathways of XN after oral administration to rats. Herein, a UHPLC-Q-Exactive Orbitrap MS was adopted for the potential metabolites detection. A stepwise targeted matching strategy for the overall identification of XN metabolites was proposed. A metabolic net (53 metabolites included) on XN in vivo and in vitro, as well as the metabolic profile investigation, were designed, preferably characterizing XN metabolites in rat plasma, urine, liver, liver microsomes, and feces. On the basis of a stepwise targeted matching strategy, the net showed that major in vivo metabolic pathways of XN in rats include glucuronidation, sulfation, methylation, demethylation, hydrogenation, dehydrogenation, hydroxylation, and so on. The proposed metabolic pathways in this research will provide essential data for further pharmaceutical studies of prenylated flavonoids and lay the foundation for further toxicity and safety studies.